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Comprehensive identification and profiling of host miRNAs in response to Singapore grouper iridovirus (SGIV) infection in grouper (Epinephelus coioides)
Guo, Chuanyu; Cui, Huachun; Ni, Songwei; Yan, Yang; Qin, Qiwei; yanyang@scsio.ac.cn; qinqw@scsio.ac.cn
2015
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号52期号:2页码:226-235
摘要microRNAs (miRNAs) are an evolutionarily conserved class of non-coding RNA molecules that participate in various biological processes. Employment of high-throughput screening strategies greatly prompts the investigation and profiling of miRNAs in diverse species. In recent years, grouper (Epinephelus spp.) aquaculture was severely affected by iridoviral diseases. However, knowledge regarding the host immune responses to viral infection, especially the miRNA-mediated immune regulatory roles, is rather limited. In this study, by employing Solexa deep sequencing approach, we identified 116 grouper miRNAs from grouper spleen-derived cells (GS). As expected, these miRNAs shared high sequence similarity with miRNAs identified in zebrafish (Danio rerio), pufferfish (Fugu rubripes), and other higher vertebrates. In the process of Singapore grouper iridovirus (SGIV) infection, 45 and 43 miRNAs with altered expression (>1.5-fold) were identified by miRNA microarray assays in grouper spleen tissues and GS cells, respectively. Furthermore, target prediction revealed 189 putative targets of these grouper miRNAs. (C) 2015 Elsevier Ltd. All rights reserved.
部门归属[Guo, Chuanyu; Ni, Songwei; Yan, Yang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Guo, Chuanyu; Ni, Songwei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Cui, Huachun] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA ; LTO
关键词Micrornas Grouper Sgiv Deep Sequencing Mirna Microarray Functional Annotation
学科领域Immunology ; Zoology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14914
专题热带海洋环境国家重点实验室(LTO)
通讯作者yanyang@scsio.ac.cn; qinqw@scsio.ac.cn
推荐引用方式
GB/T 7714		 Guo, Chuanyu,Cui, Huachun,Ni, Songwei,et al. Comprehensive identification and profiling of host miRNAs in response to Singapore grouper iridovirus (SGIV) infection in grouper (Epinephelus coioides)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,52(2):226-235.
APA Guo, Chuanyu.,Cui, Huachun.,Ni, Songwei.,Yan, Yang.,Qin, Qiwei.,...&qinqw@scsio.ac.cn.(2015).Comprehensive identification and profiling of host miRNAs in response to Singapore grouper iridovirus (SGIV) infection in grouper (Epinephelus coioides).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,52(2),226-235.
MLA Guo, Chuanyu,et al."Comprehensive identification and profiling of host miRNAs in response to Singapore grouper iridovirus (SGIV) infection in grouper (Epinephelus coioides)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 52.2(2015):226-235.
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