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A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079
Chan, Zhuhua; Zhong, Tianhua; Yi, Zhiwei; Xiao, Jing; Zeng, Runying; zeng@tio.org.cn
2015
Source PublicationBIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume20Issue:5Pages:908-914
AbstractThe aim of this work is to enhance enduracidin production by Streptomyces sp. MC079. Based on the time course analysis of the specific cell growth rate and specific enduracidin formation rate, a two-stage pH control strategy was proposed to improve enduracidin production by shifting the culture pH from 5.5 to 5.8 after 112 h of cultivation. By applying this pH control strategy, enduracidin concentration and productivity was 51.2 and 65.0% higher than results with uncontrolled pH batch fermentation. For further enhancement of enduracidin production, the effects of constant-rate feeding and pH-shift feeding strategy were investigated. The results indicated that the pH-shift feeding strategy increased the maximum concentration and productivity of enduracidin to 61.37 mg/L and 0.697 mg/L/h in the constant-rate feeding fermentation process. This is 73.3 and 88.9% higher than results with uncontrolled pH batch fermentation, respectively. The obtained optimal pH shift feeding strategy may be useful for the industrial-scale microbial production of enduracidin.
Department[Chan, Zhuhua; Zhong, Tianhua; Yi, Zhiwei; Xiao, Jing; Zeng, Runying] SOA, Inst Oceanog 3, State Key Lab Breeding Base Marine Genet Resource, Xiamen 361005, Peoples R China; [Zeng, Runying] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510000, Guangdong, Peoples R China ; LTO
KeywordStreptomyces Sp Mc079 Enduracidin Ph-shift Feeding Strategy Fed-batch Fermentation
Subject AreaBiotechnology & Applied Microbiology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14916
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorzeng@tio.org.cn
Recommended Citation
GB/T 7714
Chan, Zhuhua,Zhong, Tianhua,Yi, Zhiwei,et al. A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2015,20(5):908-914.
APA Chan, Zhuhua,Zhong, Tianhua,Yi, Zhiwei,Xiao, Jing,Zeng, Runying,&zeng@tio.org.cn.(2015).A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,20(5),908-914.
MLA Chan, Zhuhua,et al."A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 20.5(2015):908-914.
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