A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079
Chan, Zhuhua; Zhong, Tianhua; Yi, Zhiwei; Xiao, Jing; Zeng, Runying; zeng@tio.org.cn
2015
发表期刊BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷号20期号:5页码:908-914
摘要The aim of this work is to enhance enduracidin production by Streptomyces sp. MC079. Based on the time course analysis of the specific cell growth rate and specific enduracidin formation rate, a two-stage pH control strategy was proposed to improve enduracidin production by shifting the culture pH from 5.5 to 5.8 after 112 h of cultivation. By applying this pH control strategy, enduracidin concentration and productivity was 51.2 and 65.0% higher than results with uncontrolled pH batch fermentation. For further enhancement of enduracidin production, the effects of constant-rate feeding and pH-shift feeding strategy were investigated. The results indicated that the pH-shift feeding strategy increased the maximum concentration and productivity of enduracidin to 61.37 mg/L and 0.697 mg/L/h in the constant-rate feeding fermentation process. This is 73.3 and 88.9% higher than results with uncontrolled pH batch fermentation, respectively. The obtained optimal pH shift feeding strategy may be useful for the industrial-scale microbial production of enduracidin.
部门归属[Chan, Zhuhua; Zhong, Tianhua; Yi, Zhiwei; Xiao, Jing; Zeng, Runying] SOA, Inst Oceanog 3, State Key Lab Breeding Base Marine Genet Resource, Xiamen 361005, Peoples R China; [Zeng, Runying] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510000, Guangdong, Peoples R China ; LTO
关键词Streptomyces Sp Mc079 Enduracidin Ph-shift Feeding Strategy Fed-batch Fermentation
学科领域Biotechnology & Applied Microbiology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14916
专题热带海洋环境国家重点实验室(LTO)
通讯作者zeng@tio.org.cn
推荐引用方式
GB/T 7714
Chan, Zhuhua,Zhong, Tianhua,Yi, Zhiwei,et al. A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2015,20(5):908-914.
APA Chan, Zhuhua,Zhong, Tianhua,Yi, Zhiwei,Xiao, Jing,Zeng, Runying,&zeng@tio.org.cn.(2015).A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,20(5),908-914.
MLA Chan, Zhuhua,et al."A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 20.5(2015):908-914.
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