Molecular cloning and expression analysis of scd1 gene from large yellow croaker Larimichthys crocea under cold stress
Xu, Hao; Zhang, Dong Ling; Yu, Da Hui; Lv, Chang Huan; Luo, Hui Yu; Wang, Zhi Yong; zywang@jmu.edu.cn
2015
发表期刊GENE
卷号568期号:1页码:100-108
摘要Desaturation of fatty acids is an important adaptation mechanism to maintain membrane fluidity under cold stress. To comprehend the mechanism of adaptation to low temperatures in fish, we investigated stearoyl-CoA desaturase 1 (SCD1) endocrine expression in the process of cold acclimation from 15 degrees C to 7 degrees C in Larimichthys crocea. The cDNA and genomic sequences of scd1 were cloned and characterized and named as Lcscd1. The cDNA encoded an iron-containing protein of 337 amino acids with functional motifs. The full-length genome sequence of Lcscd1 was composed of 2556 nucleotides, including five exons and four introns. Tissue expression profiles by qPCR and western blot analysis revealed that Lcscd1 was highly expressed in the liver, followed by the brain. The expression of Lcscd1 mRNA in the liver was firstly down-regulated from 15 degrees C to 11 degrees C, and then up-regulated until the first day of 7 degrees C, followed by a decline until the last day. In the brain, the expression showed no significant change from 15 degrees C to 9 degrees C, but then significantly increased until the last day of 7 degrees C. SCD1 protein expression in the liver decreased from 15 degrees C to the first day of 7 degrees C, and then gradually recovered to the starting level. In the brain, SCD1 protein expression maintained rising trends in the whole process. Immunoelectron microscopic analysis showed that SCD1 was localized in fat granules, mitochondria and granular endoplasmic reticulum of hepatic cells, but only in mitochondria of encephalic cells. The results above suggested that SCD1 expression was responsive to both cold and starvation stresses in the liver, but only to cold stress in the brain. In conclusion, these findings suggested that SCD1 may be involved in fish adaptation to cold stress. (C) 2015 Elsevier B.V. All rights reserved.
部门归属[Xu, Hao; Zhang, Dong Ling; Lv, Chang Huan; Luo, Hui Yu; Wang, Zhi Yong] Jimei Univ, Coll Fisheries, Minist Agr, Key Lab Hlth Mariculture East China Sea, Xiamen 361021, Fujian Province, Peoples R China; [Yu, Da Hui] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China ; LTO
关键词Cold Challenge Stearoyl-coa Desaturase (Scd) Larimichthys Crocea
学科领域Genetics & Heredity
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14932
专题热带海洋环境国家重点实验室(LTO)
通讯作者zywang@jmu.edu.cn
推荐引用方式
GB/T 7714
Xu, Hao,Zhang, Dong Ling,Yu, Da Hui,et al. Molecular cloning and expression analysis of scd1 gene from large yellow croaker Larimichthys crocea under cold stress[J]. GENE,2015,568(1):100-108.
APA Xu, Hao.,Zhang, Dong Ling.,Yu, Da Hui.,Lv, Chang Huan.,Luo, Hui Yu.,...&zywang@jmu.edu.cn.(2015).Molecular cloning and expression analysis of scd1 gene from large yellow croaker Larimichthys crocea under cold stress.GENE,568(1),100-108.
MLA Xu, Hao,et al."Molecular cloning and expression analysis of scd1 gene from large yellow croaker Larimichthys crocea under cold stress".GENE 568.1(2015):100-108.
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