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Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response
Chen, Ting; Ren, Chunhua; Li, Wuhu; Jiang, Xiao; Xia, Jianjun; Wong, Nai-Kei; Hu, Chaoqun; chan1010@scsio.ac.cn; rosemary166@sina.com; liwuhusunny@163.com; jiangxiao4367@126.com; jianjunxia@scsio.ac.cn; wongnk@gmail.com; hucq@scsio.ac.cn
2015
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume45Issue:2Pages:231-238
AbstractCalmodulin (CaM) is an essential second messenger protein that transduces calcium signals by binding calcium ions (Ca2+) and modulating its interactions with various target proteins. In contrast to vertebrates, where CaM is well established as a cofactor for Ca2+-dependent physiological and cellular functions including host defense, there is a paucity of understanding on CaM in invertebrates (such as echinoderms) in response to immune challenge or microbial infections. In this study, we obtained and described the gene sequence of CaM from the tropical sea cucumber Stichopus monotuberculatus, a promising yet poorly characterized aquacultural species. mRNA expression of StmCaM could be detected in the intestine and coelomic fluid after Vibrio alginolyticus injection. Transcriptional and translational expression of StmCaM was inducible in nature, as evidenced by the expression patterns in primary coelomocytes following Vibrio challenge. This response could be mimicked by the Vibrio cells membrane components or lipopolysaccharides (LPS), and blocked by co-treatment of the LPS-neutralizing agent polymyxin B (PMB). Furthermore, inhibition of CaM activity by incubation with its inhibitor tri-fluoroperazine dihydrochloride (TIP) blunted the production of Vibrio-induced nitric oxide (NO) and augmented the survival of invading Vibrio in coelomocytes. Collectively, our study here supplied the first evidence for echinoderm CaM participation in innate immunity, and provided a functional link between CaM expression and antibacterial NO production in sea cucumber. (C) 2015 Elsevier Ltd. All rights reserved.
Department[Chen, Ting; Ren, Chunhua; Li, Wuhu; Jiang, Xiao; Xia, Jianjun; Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou, Guangdong, Peoples R China; [Chen, Ting; Ren, Chunhua; Li, Wuhu; Jiang, Xiao; Xia, Jianjun; Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Chinese Acad Sci LAMB, Guangzhou, Guangdong, Peoples R China; [Li, Wuhu] Univ Chinese Acad Sci, Beijing, Peoples R China; [Wong, Nai-Kei] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China ; LTO
KeywordCalmodulin Gene Structure Stichopus Monotuberculatus Nitric Oxide Invertebrate Innate Immunity Vibrio
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14940
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorchan1010@scsio.ac.cn; rosemary166@sina.com; liwuhusunny@163.com; jiangxiao4367@126.com; jianjunxia@scsio.ac.cn; wongnk@gmail.com; hucq@scsio.ac.cn
Recommended Citation
GB/T 7714
Chen, Ting,Ren, Chunhua,Li, Wuhu,et al. Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):231-238.
APA Chen, Ting.,Ren, Chunhua.,Li, Wuhu.,Jiang, Xiao.,Xia, Jianjun.,...&hucq@scsio.ac.cn.(2015).Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response.FISH & SHELLFISH IMMUNOLOGY,45(2),231-238.
MLA Chen, Ting,et al."Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):231-238.
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