SCSIO OpenIR  > 热带海洋环境国家重点实验室(LTO)
Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas
Xu, Fengjiao; Zhang, Yang; Li, Jun; Zhang, Yuehuan; Xiang, Zhiming; Yu, Ziniu; yzhang@scsio.ac.cn
2015
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume45Issue:2Pages:510-516
AbstractMyeloid differentiation factor 88 (MyD88) is the classic signaling adaptor that mediates Toll/interleukin-1 receptor (TIR/IL-1R) dependent activation of nuclear factor-kappa B (NF-kappa B). In this study, two naturally truncated MyD88 members were identified from the Pacific oyster (Crassostrea gigas), namely CgMyD88-T1 and CgMyD88-T2. The full-length cDNA of CgMyD88-T1, CgMyD88-T2 are 976 bp and 1038 bp in length, containing an ORF of 552 bp and 555 bp, respectively. The two ORF encode a putative protein of 183 and 184 amino acids, respectively, with a calculated molecular weight of about 21 and 22 kDa. When compared to complete MyD88 paralogues, we found that both CgMyD88-T1 and CgMyD88-T2 contain only TIR domain but lack DD (Death Domain), which share 90.8% of similarity and 71.7% of identity with each other. Phylogenetic tree demonstrated that CgMyD88-T1 and CgMyD88-T2 clustered together and belonged to mollusk branch. Meanwhile, genomic arrangement analysis displayed that the two truncated MyD88s were distributed in tandem in one scaffold, revealing that they may originate from one truncated MyD88 ancestor recently. Expression profile showed that both of CgMyD88 variants were ubiquitously expressed in all tested tissues with highest expression in the gills and hemocytes, respectively. Both truncated CgMyD88 mRNAs were significantly up-regulated in hemocytes under HKLM (heat-killed Listeria monocytogenes) and HKVA (heat-killed Vibrio alginolyticus) challenge. Moreover, either CgMyD88-T1 or CgMyD88-T2 were able to inhibit MyD88 activated Rel/NF-kappa B activity in HEK293 cell, demonstrating their negative role in regulating MyD88-mediated immune signaling. (C) 2015 Elsevier Ltd. All rights reserved.
Department[Xu, Fengjiao; Zhang, Yang; Li, Jun; Zhang, Yuehuan; Xiang, Zhiming; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Xu, Fengjiao] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LTO
KeywordCrassostrea Gigas Truncated Myd88 Variants Rel/nf-kappa b Tir Domain
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14941
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authoryzhang@scsio.ac.cn
Recommended Citation
GB/T 7714
Xu, Fengjiao,Zhang, Yang,Li, Jun,et al. Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):510-516.
APA Xu, Fengjiao.,Zhang, Yang.,Li, Jun.,Zhang, Yuehuan.,Xiang, Zhiming.,...&yzhang@scsio.ac.cn.(2015).Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,45(2),510-516.
MLA Xu, Fengjiao,et al."Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):510-516.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.