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Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities
Wan Peng; Yang Xiaoman; Cai Bingna; Chen Hua; Sun Huili; Chen Deke; Pan Jianyu; jypan@scsio.ac.cn
2015
Source PublicationJOURNAL OF OCEAN UNIVERSITY OF CHINA
Volume14Issue:4Pages:651-662
AbstractIn the present study, ultrasonic extraction technique (UET) is used to improve the yield of polysaccharides from Laminaria japonica (LJPs). And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design (BBD) combined with response surface methodology (RSM) is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80A degrees C and ratio of material to solvent at 1:50 (g mL(-1)). Under these optimal ultrasonic extraction conditions, an actual experimental yield (5.75% +/- 0.3%) is close to the predicted result (5.67%) with no significant difference (P > 0.05). Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides (LJP) and two major ethanol precipitated fractions (LJP1 and LJP2) are in a concentration-dependent manner. LJP2 (30%-60% ethanol precipitated polysaccharides) possesses the strongest alpha-glucosidase inhibitory activity and moderate scavenging activity against hydroxyl radicals (66.09% +/- 2.19%, 3.0 mg mL(-1)). Also, the inhibitory activity against alpha-glucosidase (59.08% +/- 3.79%, 5.0 mg mL(-1)) is close to that of acarbose (63.99% +/- 3.27%, 5.0 mg mL(-1)). LJP1 (30% ethanol precipitated polysaccharides) exhibits the strongest scavenging activity against hydroxyl radicals (99.80% +/- 0.00%, 3.0 mg mL(-1)) and moderate alpha-glucosidase inhibitory activity (47.76% +/- 1.92%, 5.0 mg mL(-1)). LJP shows the most remarkable DPPH scavenging activity (66.20% +/- 0.11%, 5.0 mg mL(-1)) but weakest alpha-glucosidase inhibitory activity (37.77% +/- 1.30%, 5.0 mg mL(-1)). However, all these LJPs exert weak inhibitory effects against alpha-amylase. These results show that UET is an effective method for extracting bioactive polysaccharides from seaweed materials. LJP1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of diabetes. This study provides scientific evidence and advances in the preparation technology and a hypoglycemic activities evaluation method for seaweed polysaccharides, especially glycosidase inhibition in combination with an antioxidative activity evaluation method.
Department[Wan Peng; Cai Bingna; Chen Hua; Sun Huili; Chen Deke; Pan Jianyu] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Wan Peng; Chen Deke] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yang Xiaoman] Ocean Univ China, Qingdao 266100, Peoples R China ; LTO
KeywordPolysaccharides Laminaria Japonica Ultrasonic Extraction Antioxidative Alpha-glucosidase Alpha-amylase
Subject AreaOceanography
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14951
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorjypan@scsio.ac.cn
Recommended Citation
GB/T 7714
Wan Peng,Yang Xiaoman,Cai Bingna,et al. Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2015,14(4):651-662.
APA Wan Peng.,Yang Xiaoman.,Cai Bingna.,Chen Hua.,Sun Huili.,...&jypan@scsio.ac.cn.(2015).Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities.JOURNAL OF OCEAN UNIVERSITY OF CHINA,14(4),651-662.
MLA Wan Peng,et al."Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities".JOURNAL OF OCEAN UNIVERSITY OF CHINA 14.4(2015):651-662.
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