Transcriptome and Molecular Pathway Analysis of the Hepatopancreas in the Pacific White Shrimp Litopenaeus vannamei under Chronic Low-Salinity Stress
Chen, Ke; Li, Erchao; Li, Tongyu; Xu, Chang; Wang, Xiaodan; Lin, Heizhao; Qin, Jian G.; Chen, Liqiao; ecli@bio.ecnu.edu.cn; lqchen@bio.ecnu.edu.cn
2015
发表期刊PLOS ONE
卷号10期号:7页码:e0131503-
摘要The Pacific white shrimp Litopenaeus vannamei is a euryhaline penaeid species that shows ontogenetic adaptations to salinity, with its larvae inhabiting oceanic environments and postlarvae and juveniles inhabiting estuaries and lagoons. Ontogenetic adaptations to salinity manifest in L. vannamei through strong hyper-osmoregulatory and hypo-osmoregulatory patterns and an ability to tolerate extremely low salinity levels. To understand this adaptive mechanism to salinity stress, RNA-seq was used to compare the transcriptomic response of L. vannamei to changes in salinity from 30 (control) to 3 practical salinity units (psu) for 8 weeks. In total, 26,034 genes were obtained from the hepatopancreas tissue of L. vannamei using the Illumina HiSeq 2000 system, and 855 genes showed significant changes in expression under salinity stress. Eighteen top Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly involved in physiological responses, particularly in lipid metabolism, including fatty-acid biosynthesis, arachidonic acid metabolism and glycosphingolipid and glycosaminoglycan metabolism. Lipids or fatty acids can reduce osmotic stress in L. vannamei by providing additional energy or changing the membrane structure to allow osmoregulation in relevant organs, such as the gills. Steroid hormone biosynthesis and the phosphonate and phosphinate metabolism pathways were also involved in the adaptation of L. vannamei to low salinity, and the differential expression patterns of 20 randomly selected genes were validated by quantitative real-time PCR (qPCR). This study is the first report on the long-term adaptive transcriptomic response of L. vannamei to low salinity, and the results will further our understanding of the mechanisms underlying osmoregulation in euryhaline crustaceans.
部门归属[Chen, Ke; Li, Erchao; Li, Tongyu; Xu, Chang; Wang, Xiaodan; Chen, Liqiao] E China Normal Univ, Lab Aquaculture Nutr & Environm Hlth, Sch Life Sci, Shanghai 200062, Peoples R China; [Lin, Heizhao] Shenzhen Base South China Sea Fisheries Res Inst, Shenzhen, Peoples R China; [Qin, Jian G.] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia ; LTO
学科领域Science & Technology - Other Topics
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14960
专题热带海洋环境国家重点实验室(LTO)
通讯作者ecli@bio.ecnu.edu.cn; lqchen@bio.ecnu.edu.cn
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Chen, Ke,Li, Erchao,Li, Tongyu,et al. Transcriptome and Molecular Pathway Analysis of the Hepatopancreas in the Pacific White Shrimp Litopenaeus vannamei under Chronic Low-Salinity Stress[J]. PLOS ONE,2015,10(7):e0131503-.
APA Chen, Ke.,Li, Erchao.,Li, Tongyu.,Xu, Chang.,Wang, Xiaodan.,...&lqchen@bio.ecnu.edu.cn.(2015).Transcriptome and Molecular Pathway Analysis of the Hepatopancreas in the Pacific White Shrimp Litopenaeus vannamei under Chronic Low-Salinity Stress.PLOS ONE,10(7),e0131503-.
MLA Chen, Ke,et al."Transcriptome and Molecular Pathway Analysis of the Hepatopancreas in the Pacific White Shrimp Litopenaeus vannamei under Chronic Low-Salinity Stress".PLOS ONE 10.7(2015):e0131503-.
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