Singapore grouper iridovirus (SGIV) encoded SGIV-miR-13 attenuates viral infection via modulating major capsid protein expression
Yan, Yang; Guo, Chuanyu; Ni, Songwei; Wei, Jingguang; Li, Pengfei; Wei, Shina; Cui, Huachun; Qin, Qiwei; qinqw@scsio.ac.cn
2015
发表期刊VIRUS RESEARCH
卷号205页码:45-53
摘要Singapore grouper iridovirus (SGIV) encodes a number of microRNAs (miRNAs) during infection. Among these, SGIV-miR-13 has robust expression at early stage after SGIV inoculation, raising a huge possibility that it participates in the viral infection. In the present study, we found that SGIV-miR-13 overexpression led to a significant reduction in viral load in cultured fish cells with SGIV infection, as demonstrated by less level of viral transcripts, viral-induced cytopathic effect (CPE) and assembled viral particles. In silica analysis showed that SGIV-miR-13 maps antisense to the coding region of SGIV major capsid protein (SGIV-MCP), suggesting it to be a potential target of SGIV-miR-13. Coincidently, SGIV-miR-13 showed an inverted expression profile with SGIV-MCP during SGIV infection, and luciferase reporter assay further demonstrated SGIV-MCP as the direct target of SGIV-miR-13. Functionally, overexpression of SGIV-miR-13 inhibited, whereas knockdown of SGIV-miR-13 restored the expression of SGIV-MCP during viral infection, resulting in altered viral progeny emergences. In conclusion, our data suggest that SGIV-miR-13 functions in a negative regulatory mechanism to restrict early viral replication, and thus prevents excessive cellular antiviral responses during SGIV infection. The detailed investigation of SGIV encoded miRNAs may provide new insights into the mechanism of iridovirus pathogenesis. (C) 2015 Elsevier B.V. All rights reserved.
部门归属[Yan, Yang; Guo, Chuanyu; Ni, Songwei; Wei, Jingguang; Li, Pengfei; Wei, Shina; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Cui, Huachun] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA; [Guo, Chuanyu; Ni, Songwei; Li, Pengfei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LTO
关键词Singapore Grouper Iridovirus Microrna Sgiv-mir-13 Major Capsid Protein Viral Infection
学科领域Virology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14961
专题热带海洋环境国家重点实验室(LTO)
通讯作者qinqw@scsio.ac.cn
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Yan, Yang,Guo, Chuanyu,Ni, Songwei,et al. Singapore grouper iridovirus (SGIV) encoded SGIV-miR-13 attenuates viral infection via modulating major capsid protein expression[J]. VIRUS RESEARCH,2015,205:45-53.
APA Yan, Yang.,Guo, Chuanyu.,Ni, Songwei.,Wei, Jingguang.,Li, Pengfei.,...&qinqw@scsio.ac.cn.(2015).Singapore grouper iridovirus (SGIV) encoded SGIV-miR-13 attenuates viral infection via modulating major capsid protein expression.VIRUS RESEARCH,205,45-53.
MLA Yan, Yang,et al."Singapore grouper iridovirus (SGIV) encoded SGIV-miR-13 attenuates viral infection via modulating major capsid protein expression".VIRUS RESEARCH 205(2015):45-53.
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