Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response
Chen, Ting; Ren, Chunhua; Li, Wuhu; Jiang, Xiao; Xia, Jianjun; Wong, Nai-Kei; Hu, Chaoqun; chan1010@scsio.ac.cn; rosemary166@sina.com; liwuhusunny@163.com; jiangxiao4367@126.com; jianjunxia@scsio.ac.cn; wongnk@gmail.com; hucq@scsio.ac.cn
2015
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号45期号:2页码:231-238
文章类型1050-4648
摘要Calmodulin (CaM) is an essential second messenger protein that transduces calcium signals by binding calcium ions (Ca2+) and modulating its interactions with various target proteins. In contrast to vertebrates, where CaM is well established as a cofactor for Ca2+-dependent physiological and cellular functions including host defense, there is a paucity of understanding on CaM in invertebrates (such as echinoderms) in response to immune challenge or microbial infections. In this study, we obtained and described the gene sequence of CaM from the tropical sea cucumber Stichopus monotuberculatus, a promising yet poorly characterized aquacultural species. mRNA expression of StmCaM could be detected in the intestine and coelomic fluid after Vibrio alginolyticus injection. Transcriptional and translational expression of StmCaM was inducible in nature, as evidenced by the expression patterns in primary coelomocytes following Vibrio challenge. This response could be mimicked by the Vibrio cells membrane components or lipopolysaccharides (LPS), and blocked by co-treatment of the LPS-neutralizing agent polymyxin B (PMB). Furthermore, inhibition of CaM activity by incubation with its inhibitor tri-fluoroperazine dihydrochloride (TIP) blunted the production of Vibrio-induced nitric oxide (NO) and augmented the survival of invading Vibrio in coelomocytes. Collectively, our study here supplied the first evidence for echinoderm CaM participation in innate immunity, and provided a functional link between CaM expression and antibacterial NO production in sea cucumber. (C) 2015 Elsevier Ltd. All rights reserved.
部门归属[Chen, Ting; Ren, Chunhua; Li, Wuhu; Jiang, Xiao; Xia, Jianjun; Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou, Guangdong, Peoples R China; [Chen, Ting; Ren, Chunhua; Li, Wuhu; Jiang, Xiao; Xia, Jianjun; Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Chinese Acad Sci LAMB, Guangzhou, Guangdong, Peoples R China; [Li, Wuhu] Univ Chinese Acad Sci, Beijing, Peoples R China; [Wong, Nai-Kei] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China ; LTO
关键词Calmodulin Gene Structure Stichopus Monotuberculatus Nitric Oxide Invertebrate Innate Immunity Vibrio
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别SCI
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15053
专题热带海洋环境国家重点实验室(LTO)
通讯作者chan1010@scsio.ac.cn; rosemary166@sina.com; liwuhusunny@163.com; jiangxiao4367@126.com; jianjunxia@scsio.ac.cn; wongnk@gmail.com; hucq@scsio.ac.cn
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Chen, Ting,Ren, Chunhua,Li, Wuhu,et al. Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):231-238.
APA Chen, Ting.,Ren, Chunhua.,Li, Wuhu.,Jiang, Xiao.,Xia, Jianjun.,...&hucq@scsio.ac.cn.(2015).Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response.FISH & SHELLFISH IMMUNOLOGY,45(2),231-238.
MLA Chen, Ting,et al."Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):231-238.
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