Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas
Xu, Fengjiao; Zhang, Yang; Li, Jun; Zhang, Yuehuan; Xiang, Zhiming; Yu, Ziniu; yzhang@scsio.ac.cn
2015
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号45期号:2页码:510-516
文章类型1050-4648
摘要Myeloid differentiation factor 88 (MyD88) is the classic signaling adaptor that mediates Toll/interleukin-1 receptor (TIR/IL-1R) dependent activation of nuclear factor-kappa B (NF-kappa B). In this study, two naturally truncated MyD88 members were identified from the Pacific oyster (Crassostrea gigas), namely CgMyD88-T1 and CgMyD88-T2. The full-length cDNA of CgMyD88-T1, CgMyD88-T2 are 976 bp and 1038 bp in length, containing an ORF of 552 bp and 555 bp, respectively. The two ORF encode a putative protein of 183 and 184 amino acids, respectively, with a calculated molecular weight of about 21 and 22 kDa. When compared to complete MyD88 paralogues, we found that both CgMyD88-T1 and CgMyD88-T2 contain only TIR domain but lack DD (Death Domain), which share 90.8% of similarity and 71.7% of identity with each other. Phylogenetic tree demonstrated that CgMyD88-T1 and CgMyD88-T2 clustered together and belonged to mollusk branch. Meanwhile, genomic arrangement analysis displayed that the two truncated MyD88s were distributed in tandem in one scaffold, revealing that they may originate from one truncated MyD88 ancestor recently. Expression profile showed that both of CgMyD88 variants were ubiquitously expressed in all tested tissues with highest expression in the gills and hemocytes, respectively. Both truncated CgMyD88 mRNAs were significantly up-regulated in hemocytes under HKLM (heat-killed Listeria monocytogenes) and HKVA (heat-killed Vibrio alginolyticus) challenge. Moreover, either CgMyD88-T1 or CgMyD88-T2 were able to inhibit MyD88 activated Rel/NF-kappa B activity in HEK293 cell, demonstrating their negative role in regulating MyD88-mediated immune signaling. (C) 2015 Elsevier Ltd. All rights reserved.
部门归属[Xu, Fengjiao; Zhang, Yang; Li, Jun; Zhang, Yuehuan; Xiang, Zhiming; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Xu, Fengjiao] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LTO
关键词Crassostrea Gigas Truncated Myd88 Variants Rel/nf-kappa b Tir Domain
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别SCI
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15054
专题热带海洋环境国家重点实验室(LTO)
通讯作者yzhang@scsio.ac.cn
推荐引用方式
GB/T 7714
Xu, Fengjiao,Zhang, Yang,Li, Jun,et al. Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):510-516.
APA Xu, Fengjiao.,Zhang, Yang.,Li, Jun.,Zhang, Yuehuan.,Xiang, Zhiming.,...&yzhang@scsio.ac.cn.(2015).Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,45(2),510-516.
MLA Xu, Fengjiao,et al."Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):510-516.
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