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Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation
Deng, Si-ping; Zhu, Chun-hua; Sun, Jing; Wang, Wen-da; Wu, Tian-li; Chen, Hua-pu; Shi, Shang-li; Li, Guang-li; guangli211@163.com
2015
Source PublicationGENES & GENOMICS
Volume37Issue:8Pages:669-677
Subtype1976-9571
AbstractWinged helix/forkhead transcription factor gene 2 (Foxl2) plays a crucial role during early ovarian development in fish. Sex steroids and aromatase inhibitors can regulate gonadal differentiation in teleosts. To address the role of Foxl2 in gonadal differentiation, foxl2 was isolated and characterized from the Hong Kong catfish, Clarias fuscus. Tissue distribution analysis by quantitative real-time PCR (qPCR) showed that the foxl2 mRNA was highest in the ovaries; moderate in the female pituitary and hypothalamus; but weak in the forebrain, liver, testis and male pituitary, with a sexually dimorphic pattern in which there was higher expression overall in females versus males. Treatment of the fries during the period of gonadal differentiation [2-30 days post hatching (dph)] with 17 alpha-methyltestosterone (MT), an aromatase inhibitor (letrozole) and 17 beta-estradiol (E-2) affected the expression of foxl2. The expression of foxl2 was highest before gonadal differentiation (12 dph), but decreased significantly after gonadal differentiation (18-30 dph). In addition, MT and letrozole were able to down-regulate foxl2, but E-2 up-regulated foxl2 during gonadal morphological differentiation. These results indicate the significant roles of Foxl2 in the gonadal differentiation of C. fuscus.
Department[Deng, Si-ping; Zhu, Chun-hua; Sun, Jing; Wang, Wen-da; Wu, Tian-li; Chen, Hua-pu; Shi, Shang-li; Li, Guang-li] Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China; [Deng, Si-ping; Zhu, Chun-hua; Sun, Jing; Wang, Wen-da; Wu, Tian-li; Chen, Hua-pu; Shi, Shang-li; Li, Guang-li] Guangdong Ocean Univ, Guangdong Higher Educ Inst, Key Lab Aquaculture South China Sea Aquat Econ An, Zhanjiang 524088, Peoples R China ; LTO
KeywordMethyltestosterone Estradiol Letrozole Clarias Fuscus Foxl2
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
Indexed BySCI
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15062
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorguangli211@163.com
Recommended Citation
GB/T 7714
Deng, Si-ping,Zhu, Chun-hua,Sun, Jing,et al. Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation[J]. GENES & GENOMICS,2015,37(8):669-677.
APA Deng, Si-ping.,Zhu, Chun-hua.,Sun, Jing.,Wang, Wen-da.,Wu, Tian-li.,...&guangli211@163.com.(2015).Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation.GENES & GENOMICS,37(8),669-677.
MLA Deng, Si-ping,et al."Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation".GENES & GENOMICS 37.8(2015):669-677.
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