Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities
Wan Peng; Yang Xiaoman; Cai Bingna; Chen Hua; Sun Huili; Chen Deke; Pan Jianyu; jypan@scsio.ac.cn
2015
发表期刊JOURNAL OF OCEAN UNIVERSITY OF CHINA
卷号14期号:4页码:651-662
文章类型1672-5182
摘要In the present study, ultrasonic extraction technique (UET) is used to improve the yield of polysaccharides from Laminaria japonica (LJPs). And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design (BBD) combined with response surface methodology (RSM) is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80A degrees C and ratio of material to solvent at 1:50 (g mL(-1)). Under these optimal ultrasonic extraction conditions, an actual experimental yield (5.75% +/- 0.3%) is close to the predicted result (5.67%) with no significant difference (P > 0.05). Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides (LJP) and two major ethanol precipitated fractions (LJP1 and LJP2) are in a concentration-dependent manner. LJP2 (30%-60% ethanol precipitated polysaccharides) possesses the strongest alpha-glucosidase inhibitory activity and moderate scavenging activity against hydroxyl radicals (66.09% +/- 2.19%, 3.0 mg mL(-1)). Also, the inhibitory activity against alpha-glucosidase (59.08% +/- 3.79%, 5.0 mg mL(-1)) is close to that of acarbose (63.99% +/- 3.27%, 5.0 mg mL(-1)). LJP1 (30% ethanol precipitated polysaccharides) exhibits the strongest scavenging activity against hydroxyl radicals (99.80% +/- 0.00%, 3.0 mg mL(-1)) and moderate alpha-glucosidase inhibitory activity (47.76% +/- 1.92%, 5.0 mg mL(-1)). LJP shows the most remarkable DPPH scavenging activity (66.20% +/- 0.11%, 5.0 mg mL(-1)) but weakest alpha-glucosidase inhibitory activity (37.77% +/- 1.30%, 5.0 mg mL(-1)). However, all these LJPs exert weak inhibitory effects against alpha-amylase. These results show that UET is an effective method for extracting bioactive polysaccharides from seaweed materials. LJP1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of diabetes. This study provides scientific evidence and advances in the preparation technology and a hypoglycemic activities evaluation method for seaweed polysaccharides, especially glycosidase inhibition in combination with an antioxidative activity evaluation method.
部门归属[Wan Peng; Cai Bingna; Chen Hua; Sun Huili; Chen Deke; Pan Jianyu] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Wan Peng; Chen Deke] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yang Xiaoman] Ocean Univ China, Qingdao 266100, Peoples R China ; LTO
关键词Polysaccharides Laminaria Japonica Ultrasonic Extraction Antioxidative Alpha-glucosidase Alpha-amylase
学科领域Oceanography
收录类别SCI
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15064
专题热带海洋环境国家重点实验室(LTO)
通讯作者jypan@scsio.ac.cn
推荐引用方式
GB/T 7714
Wan Peng,Yang Xiaoman,Cai Bingna,et al. Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2015,14(4):651-662.
APA Wan Peng.,Yang Xiaoman.,Cai Bingna.,Chen Hua.,Sun Huili.,...&jypan@scsio.ac.cn.(2015).Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities.JOURNAL OF OCEAN UNIVERSITY OF CHINA,14(4),651-662.
MLA Wan Peng,et al."Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities".JOURNAL OF OCEAN UNIVERSITY OF CHINA 14.4(2015):651-662.
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