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Singapore grouper iridovirus (SGIV) encoded SGIV-miR-13 attenuates viral infection via modulating major capsid protein expression
Yan, Yang; Guo, Chuanyu; Ni, Songwei; Wei, Jingguang; Li, Pengfei; Wei, Shina; Cui, Huachun; Qin, Qiwei; qinqw@scsio.ac.cn
2015
Source PublicationVIRUS RESEARCH
Volume205Pages:45-53
Subtype0168-1702
AbstractSingapore grouper iridovirus (SGIV) encodes a number of microRNAs (miRNAs) during infection. Among these, SGIV-miR-13 has robust expression at early stage after SGIV inoculation, raising a huge possibility that it participates in the viral infection. In the present study, we found that SGIV-miR-13 overexpression led to a significant reduction in viral load in cultured fish cells with SGIV infection, as demonstrated by less level of viral transcripts, viral-induced cytopathic effect (CPE) and assembled viral particles. In silica analysis showed that SGIV-miR-13 maps antisense to the coding region of SGIV major capsid protein (SGIV-MCP), suggesting it to be a potential target of SGIV-miR-13. Coincidently, SGIV-miR-13 showed an inverted expression profile with SGIV-MCP during SGIV infection, and luciferase reporter assay further demonstrated SGIV-MCP as the direct target of SGIV-miR-13. Functionally, overexpression of SGIV-miR-13 inhibited, whereas knockdown of SGIV-miR-13 restored the expression of SGIV-MCP during viral infection, resulting in altered viral progeny emergences. In conclusion, our data suggest that SGIV-miR-13 functions in a negative regulatory mechanism to restrict early viral replication, and thus prevents excessive cellular antiviral responses during SGIV infection. The detailed investigation of SGIV encoded miRNAs may provide new insights into the mechanism of iridovirus pathogenesis. (C) 2015 Elsevier B.V. All rights reserved.
Department[Yan, Yang; Guo, Chuanyu; Ni, Songwei; Wei, Jingguang; Li, Pengfei; Wei, Shina; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Cui, Huachun] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA; [Guo, Chuanyu; Ni, Songwei; Li, Pengfei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LTO
KeywordSingapore Grouper Iridovirus Microrna Sgiv-mir-13 Major Capsid Protein Viral Infection
Subject AreaVirology
Indexed BySCI
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15074
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorqinqw@scsio.ac.cn
Recommended Citation
GB/T 7714
Yan, Yang,Guo, Chuanyu,Ni, Songwei,et al. Singapore grouper iridovirus (SGIV) encoded SGIV-miR-13 attenuates viral infection via modulating major capsid protein expression[J]. VIRUS RESEARCH,2015,205:45-53.
APA Yan, Yang.,Guo, Chuanyu.,Ni, Songwei.,Wei, Jingguang.,Li, Pengfei.,...&qinqw@scsio.ac.cn.(2015).Singapore grouper iridovirus (SGIV) encoded SGIV-miR-13 attenuates viral infection via modulating major capsid protein expression.VIRUS RESEARCH,205,45-53.
MLA Yan, Yang,et al."Singapore grouper iridovirus (SGIV) encoded SGIV-miR-13 attenuates viral infection via modulating major capsid protein expression".VIRUS RESEARCH 205(2015):45-53.
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