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A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis
Qu, Fufa; Xiang, Zhiming; Wang, Fuxuan; Zhang, Yang; Tong, Ying; Li, Jun; Zhang, Yuehuan; Yu, Ziniu; carlzyu@scsio.ac.cn
2015
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume51Issue:1Pages:194-201
Subtype0145-305X
AbstractThe transcription factor Fos is a member of one of the best-studied AP-1 sub-families and has been implicated in a wide variety of biological processes, including the regulation of apoptosis, immune responses and cytokine production. In this report, a novel mollusk Fos (referred to as ChFos) gene was cloned and characterized from the Hong Kong oyster, Crassostrea hongkongensis. The deduced ChFos protein sequence comprised 333 amino acids and shared significant homology with invertebrate homologs. Phylogenetic analysis revealed that ChFos clusters with Fos from Crassostrea gigas and Crassostrea ariakensis. Quantitative real-time PCR analysis revealed that ChFos mRNA was broadly expressed in all tested tissues and during different stages of the oyster's embryonic and larval development. In addition, the expression of ChFos mRNA was significantly up-regulated under challenge with microorganisms (Vibrio alginolyticus, Staphylococcus haemolyticus and Saccharomyces cerevisiae) and pathogen-associated molecular patterns (PAMPs: LPS, PGN and polyl:C). Moreover, fluorescence microscopy showed that ChFos protein is localized in the nucleus in HEK293T cells. Reporter assays suggested that ChFos may act as an efficient transcription activator in the regulation of AP-1-responsive gene expression through interaction with ChJun. Overall, this study presents the first experimental evidence of the presence and functional characteristics of Fos in mollusks, which reveals its involvement in host protection against immune challenge in the oyster. (C) 2015 Elsevier Ltd. All rights reserved.
Department[Qu, Fufa; Xiang, Zhiming; Wang, Fuxuan; Zhang, Yang; Tong, Ying; Li, Jun; Zhang, Yuehuan; Yu, Ziniu] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Qu, Fufa; Wang, Fuxuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LTO
KeywordC. Hongkongensis Innate Immunity Ap-1 Pathway Fos
Subject AreaImmunology ; Zoology
Indexed BySCI
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15077
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorcarlzyu@scsio.ac.cn
Recommended Citation
GB/T 7714
Qu, Fufa,Xiang, Zhiming,Wang, Fuxuan,et al. A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):194-201.
APA Qu, Fufa.,Xiang, Zhiming.,Wang, Fuxuan.,Zhang, Yang.,Tong, Ying.,...&carlzyu@scsio.ac.cn.(2015).A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),194-201.
MLA Qu, Fufa,et al."A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):194-201.
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