A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis
Qu, Fufa; Xiang, Zhiming; Wang, Fuxuan; Zhang, Yang; Tong, Ying; Li, Jun; Zhang, Yuehuan; Yu, Ziniu; carlzyu@scsio.ac.cn
2015
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号51期号:1页码:194-201
文章类型0145-305X
摘要The transcription factor Fos is a member of one of the best-studied AP-1 sub-families and has been implicated in a wide variety of biological processes, including the regulation of apoptosis, immune responses and cytokine production. In this report, a novel mollusk Fos (referred to as ChFos) gene was cloned and characterized from the Hong Kong oyster, Crassostrea hongkongensis. The deduced ChFos protein sequence comprised 333 amino acids and shared significant homology with invertebrate homologs. Phylogenetic analysis revealed that ChFos clusters with Fos from Crassostrea gigas and Crassostrea ariakensis. Quantitative real-time PCR analysis revealed that ChFos mRNA was broadly expressed in all tested tissues and during different stages of the oyster's embryonic and larval development. In addition, the expression of ChFos mRNA was significantly up-regulated under challenge with microorganisms (Vibrio alginolyticus, Staphylococcus haemolyticus and Saccharomyces cerevisiae) and pathogen-associated molecular patterns (PAMPs: LPS, PGN and polyl:C). Moreover, fluorescence microscopy showed that ChFos protein is localized in the nucleus in HEK293T cells. Reporter assays suggested that ChFos may act as an efficient transcription activator in the regulation of AP-1-responsive gene expression through interaction with ChJun. Overall, this study presents the first experimental evidence of the presence and functional characteristics of Fos in mollusks, which reveals its involvement in host protection against immune challenge in the oyster. (C) 2015 Elsevier Ltd. All rights reserved.
部门归属[Qu, Fufa; Xiang, Zhiming; Wang, Fuxuan; Zhang, Yang; Tong, Ying; Li, Jun; Zhang, Yuehuan; Yu, Ziniu] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Qu, Fufa; Wang, Fuxuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LTO
关键词C. Hongkongensis Innate Immunity Ap-1 Pathway Fos
学科领域Immunology ; Zoology
收录类别SCI
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15077
专题热带海洋环境国家重点实验室(LTO)
通讯作者carlzyu@scsio.ac.cn
推荐引用方式
GB/T 7714
Qu, Fufa,Xiang, Zhiming,Wang, Fuxuan,et al. A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):194-201.
APA Qu, Fufa.,Xiang, Zhiming.,Wang, Fuxuan.,Zhang, Yang.,Tong, Ying.,...&carlzyu@scsio.ac.cn.(2015).A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),194-201.
MLA Qu, Fufa,et al."A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):194-201.
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