SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
c-Jun N-terminal kinases 3 (JNK3) from orange-spotted grouper, Epinephelus coioides, inhibiting the replication of Singapore grouper iridovirus (SGIV) and SGIV-induced apoptosis
Guo, Minglan; Wei, Jingguang; Zhou, Yongcan; Qin, Qiwei; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume65Pages:169-181
AbstractC-Jun N-terminal kinases (JNKs), a subgroup of serine-threonine protein kinases that activated by phosphorylation, are involve in physiological and pathophysiological processes. JNK3 is one of JNK proteins involved in JNK3 signaling transduction. In the present study, two JNK3 isoforms, Ec-JNK3 X1 and Ec-JNK3 X2, were cloned from orange-spotted grouper, Epinephelus coioides. Both Ec-JNK3 X1 and Ec-JNK3 X2 were mainly expressed in liver, gill, skin, brain and muscle of juvenile grouper. The relative expression of EcANK3 X2 mRNA was much higher in muscle and gill than that of Ec-JNK3 X1. Isoform-specific immune response to challenges was revealed by the expression profiles in vivo. Immunofluorescence staining indicated that JNK3 was localized in the cytoplasm of grouper spleen (GS) cells and shown immune response to SGIV infection in vitro. Over-expressing Ec-JNK3 X1 and/or Ec-JNK3 X2 inhibited the SGIV infection and replication and the SGIV-induced apoptosis. To achieve the antiviral and anti-apoptosis activities, JNK3 promoted the activation of genes ISRE and type I IFN in the antiviral IFN signaling pathway, and inhibited the activation of transcription factors NF-kappa B and p53 relating to apoptosis, respectively. Ec-JNK3 X2 showed stronger activities in antivirus and anti-apoptosis than that of Ec-JNK3 X1. Our results not only define the characterization of JNK3 but also reveal new immune functions and the molecular mechanisms of JNK3 on iridoviruses infection and the virus-induced apoptosis. (C) 2016 Published by Elsevier Ltd.
Department[Guo, Minglan; Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Guo, Minglan; Wei, Jingguang] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Zhou, Yongcan] Haitian Univ, Minist Educ, Key Lab Trop Biol Resources, Haikou, Peoples R China ; LMB
KeywordEpinephelus Coioides C-jun N-terminal Kinases 3 Singapore Grouper Iridovirus (Sgiv) Apoptosis Antiviral Activity
Subject AreaImmunology ; Zoology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15259
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorQin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,et al. c-Jun N-terminal kinases 3 (JNK3) from orange-spotted grouper, Epinephelus coioides, inhibiting the replication of Singapore grouper iridovirus (SGIV) and SGIV-induced apoptosis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:169-181.
APA Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,Qin, Qiwei,&Qin, QW .(2016).c-Jun N-terminal kinases 3 (JNK3) from orange-spotted grouper, Epinephelus coioides, inhibiting the replication of Singapore grouper iridovirus (SGIV) and SGIV-induced apoptosis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,65,169-181.
MLA Guo, Minglan,et al."c-Jun N-terminal kinases 3 (JNK3) from orange-spotted grouper, Epinephelus coioides, inhibiting the replication of Singapore grouper iridovirus (SGIV) and SGIV-induced apoptosis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 65(2016):169-181.
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