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Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate
Wang, Yilong; Zhang, Yun; Hu, Yunfeng; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
2016
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume180Issue:6Pages:1196-1212
AbstractOne novel microbial esterase PHE21 was cloned from the genome of Pseudomonas oryzihabitans HUP022 identified from the deep sea of the Western Pacific. PHE21 was heterologously expressed and functionally characterized to be a robust esterase which behaved high resistance to various metal ions, organic solvents, surfactants, and NaCl. Despite the fact that the two enantiomers of ethyl 3-hydroxybutyrate were hard to be enzymatically resolved before, we successfully resolved racemic ethyl 3-hydroxybutyrate through direct hydrolysis reactions and generated chiral ethyl (S)-3-hydroxybutyrate using esterase PHE21. After process optimization, the enantiomeric excess, the conversion rate, and the yield of desired product ethyl (S)-3-hydroxybutyrate could reach 99, 65, and 87 %, respectively. PHE21 is a novel marine microbial esterase with great potential in asymmetric synthesis as well as in other industries.
Department[Wang, Yilong; Zhang, Yun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Yilong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhang, Yun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Hu, Yunfeng] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China ; LMB
KeywordDeep-sea Microorganism Robust Esterase Kinetic Resolution Ethyl (s)-3-hydroxybutyrate High Stereo-selectivity
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15262
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorHu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Wang, Yilong,Zhang, Yun,Hu, Yunfeng,et al. Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,180(6):1196-1212.
APA Wang, Yilong,Zhang, Yun,Hu, Yunfeng,Hu, YF ,Hu, YF ,&Hu, YF .(2016).Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,180(6),1196-1212.
MLA Wang, Yilong,et al."Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 180.6(2016):1196-1212.
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