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Antiviral function of Tachyplesin I against iridovirus and nodavirus
Xie, Haiwei; Wei, Jingguang; Qin, Qiwei; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume58Pages:96-102
AbstractAntimicrobial peptides (AMPs) are ubiquitously found in living organisms and are an important component in innate immune response. Tachyplesin I is a potent antimicrobial peptide isolated from the hemocytes of the horseshoe crab, Tachypleus tridentatus. Previous studies have shown that the 17-residue peptide exhibits a wide spectrum of antimicrobial activity against Gram-negative and Grampositive bacteria, fungi, protozoa, and viruses. However, the efficiencies and defense mechanisms of the Tachyplesin I against fish viruses are still unknown. In this study, Tachyplesin I showed a key role in inhibiting the infection and replication of two kinds of newly emerging marine fish viruses, an enveloped DNA virus of Singapore grouper iridovirus (SGIV), and a non-enveloped RNA virus of viral nervous necrosis virus (RGNNV). Synthetic peptides of Tachyplesin I incubated with virus or cells before infection reduced the viral infectivity. Synthetic peptides of Tachyplesin I drastically decreased SGIV and RGNNV titers and viral gene expression. Grouper spleen (GS) and brain (GB) cells over-expressing Tachyplesin I (GS/pcDNA3.1-flag-Tac I and GB/pcDNA3.1-flag-Tac I) support the inhibition of viral infection. Tachyplesin I activated type I IFN and Interferon-sensitive response element (ISRE) in vitro. The promoter activity of IFN-13 and ISRE were significantly up-regulated in cells transfected with pcDNA3.1-flag-Tac I after infection with SGIV and VNNV. These results suggest that Tachyplesin I is importantly involved in host immune responses to invasion of viral pathogens. (C) 2016 Elsevier Ltd. All rights reserved.
Department[Xie, Haiwei] Bengbu Coll, Dept Food & Bioengn, Bengbu 233030, Peoples R China; [Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China ; LMB
KeywordTachyplesin i Antiviral Effects Epinephelus Coioides Sgiv Rgnnv
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15264
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorQin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Xie, Haiwei,Wei, Jingguang,Qin, Qiwei,et al. Antiviral function of Tachyplesin I against iridovirus and nodavirus[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:96-102.
APA Xie, Haiwei,Wei, Jingguang,Qin, Qiwei,&Qin, QW .(2016).Antiviral function of Tachyplesin I against iridovirus and nodavirus.FISH & SHELLFISH IMMUNOLOGY,58,96-102.
MLA Xie, Haiwei,et al."Antiviral function of Tachyplesin I against iridovirus and nodavirus".FISH & SHELLFISH IMMUNOLOGY 58(2016):96-102.
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