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Genomic structure and expression pattern of MHC II alpha and II beta genes reveal an unusual immune trait in lined seahorse Hippocampus erectus
Luo, Wei; Wang, Xin; Qu, Hongyue; Qin, Geng; Zhang, Huixian; Lin, Qiang; Lin, Q (reprint author), 164 Xingang West Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume58Pages:521-529
AbstractThe major histocompatibility complex (MHC) genes are crucial in the adaptive immune system, and the gene duplication of MHC in animals can generally result in immune flexibility. In this study, we found that the lined seahorse (Hippocampus erectus) has only one gene copy number (GCN) of MHC II alpha and II beta, which is different from that in other teleosts. Together with the lack of spleen and gut-associated lymphatic tissue (GALT), the seahorse may be referred to as having a partial but natural "immunodeficiency". Highly variable amino acid residues were found in the II alpha and 1113 domains, especially in the alpha 1 and beta 1 domains with 9.62% and 8.43% allelic variation, respectively. Site models revealed seven and ten positively selected positions in the alpha 1 and beta 1 domains, respectively. Real-time PCR experiments showed high expression levels of the MHC II genes in intestine (In), gill (Gi) and trunk kidney (TK) and medium in muscle (Mu) and brood pouch (BP), and the expression levels were significantly up-regulated after bacterial infection. Specially, relative higher expression level of both MHC II alpha and II beta was found in Mu and BP when compared with other fish species, in which MHC II is expressed negligibly in Mu. These results indicate that apart from TIC, Gi and In, MU and BP play an important role in the immune response against pathogens in the seahorse. In conclusion, high allelic variation and strong positive selection in PBR and relative higher expression in MU and BP are speculated to partly compensate for the immunodeficiency. (C) 2016 Elsevier Ltd. All rights reserved.
Department[Luo, Wei; Wang, Xin; Qu, Hongyue; Qin, Geng; Zhang, Huixian; Lin, Qiang] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Xin; Qu, Hongyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LMB
KeywordMajor Histocompatibility Complex Ii Polymorphism Genomic Structure Selective Pressure Hippocampus Erectus
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15265
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorLin, Q (reprint author), 164 Xingang West Rd, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Luo, Wei,Wang, Xin,Qu, Hongyue,et al. Genomic structure and expression pattern of MHC II alpha and II beta genes reveal an unusual immune trait in lined seahorse Hippocampus erectus[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:521-529.
APA Luo, Wei.,Wang, Xin.,Qu, Hongyue.,Qin, Geng.,Zhang, Huixian.,...&Lin, Q .(2016).Genomic structure and expression pattern of MHC II alpha and II beta genes reveal an unusual immune trait in lined seahorse Hippocampus erectus.FISH & SHELLFISH IMMUNOLOGY,58,521-529.
MLA Luo, Wei,et al."Genomic structure and expression pattern of MHC II alpha and II beta genes reveal an unusual immune trait in lined seahorse Hippocampus erectus".FISH & SHELLFISH IMMUNOLOGY 58(2016):521-529.
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