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The leptin system and its expression at different nutritional and pregnant stages in lined seahorse (Hippocampus erectus)
Zhang, Huixian; Qin, Geng; Zhang, Yanhong; Li, Shuisheng; Lin, Qiang; Lin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationBIOLOGY OPEN
Volume5Issue:10Pages:1508-1515
AbstractLeptin is an essential hormone for the regulation of energy metabolism and food intake in vertebrate animals. To better understand the physiological roles of leptin in nutrient regulation in paternal ovoviviparous fish (family Syngnathidae), the present study cloned the full-length of leptin-a and leptin receptor (lepr) genes in lined seahorse (Hippocampus erectus). Results showed that there was a 576-bp intron between two exons in leptin-a gene but no leptin-b gene in seahorse. Although the primary amino acid sequence conservation of seahorse leptin-a was very low, the 3-D structure modeling of seahorse leptin-a revealed strong conservation of tertiary structure with other vertebrates. Seahorse leptin-a mRNA was highly expressed in brain, whereas lepr mRNA was mainly expressed in ovary and gill. Interestingly, both leptin-a and lepr mRNA were expressed in the brood pouch of male seahorse, suggesting the leptin system plays a role during the male pregnancy. Physiological experiments showed that the expression of hepatic leptin-a and lepr mRNA in unfed seahorses was significantly higher than that in those fed 100%, as well as 60%, of their food during the fasting stage, showing that seahorse might initiate the leptin system to regulate its energy metabolism while starving. Moreover, the expression of leptin-a in the brood pouch of pregnant seahorse was significantly upregulated compared with non-pregnant seahorse, whereas the expression of lepr was downregulated, suggesting that the leptin system might be involved in the male pregnancy. In conclusion, the leptin system plays a role in the energy metabolism and food intake, and might provide new insights into molecular regulation of male pregnancy in seahorse.
Department[Zhang, Huixian; Qin, Geng; Zhang, Yanhong; Lin, Qiang] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Li, Shuisheng] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China ; LMB
KeywordLeptin Leptin Receptor Nutrient Pregnancy Seahorse
Subject AreaLife Sciences & Biomedicine - Other Topics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15273
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorLin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Zhang, Huixian,Qin, Geng,Zhang, Yanhong,et al. The leptin system and its expression at different nutritional and pregnant stages in lined seahorse (Hippocampus erectus)[J]. BIOLOGY OPEN,2016,5(10):1508-1515.
APA Zhang, Huixian,Qin, Geng,Zhang, Yanhong,Li, Shuisheng,Lin, Qiang,&Lin, Q .(2016).The leptin system and its expression at different nutritional and pregnant stages in lined seahorse (Hippocampus erectus).BIOLOGY OPEN,5(10),1508-1515.
MLA Zhang, Huixian,et al."The leptin system and its expression at different nutritional and pregnant stages in lined seahorse (Hippocampus erectus)".BIOLOGY OPEN 5.10(2016):1508-1515.
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