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Formation of diploid and triploid hybrid groupers (hybridization of Epinephelus coioides female x Epinephelus lanceolatus male) and their 5S gene analysis
Huang, Wen; Qin, Qinbo; Yang, Huirong; Li, Shuisheng; Hu, Chaoqun; Wang, Yude; Zhang, Yong; Liu, Shaojun; Lin, Haoran; Zhang, Y (reprint author), Sun Yat Sen Univ, Inst Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China.; Zhang, Y (reprint author), Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China.; Liu, SJ (reprint author), Hunan Normal Univ, Coll Life Sci, State Educ Minist China, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China.
2016
Source PublicationBMC GENETICS
Volume17Pages:-
AbstractBackground: Interspecies hybridization is widely used to achieve heterosis or hybrid vigor, which has been observed and harnessed by breeders for centuries. Natural allopolyploid hybrids generally exhibit more superior heterosis than both the diploid progenies and their parental species. However, polyploid formation processes have been long ignored, the genetic basis of heterosis in polyploids remains elusive. Results: In the present study, triploid hybrids had been demonstrated to contain two sets of chromosomes from mother species and one set from father species. Cellular polyploidization process in the embryos had been traced. The triploid hybrids might be formed by failure formation of the second polarized genome during the second meiosis stage. Four spindle centers were observed in anaphase stage of the first cell division. Three spindle centers were observed in side of cell plate after the first cell division. The 5S rDNA genes of four types of groupers were cloned and analyzed. The diploid and triploid hybrids had been proved to contain the tandem chimera structures which were recombined by maternal and paternal monomer units. The results indicated that genome re-fusion had occurred in the hybrid progenies. To further elucidate the genetic patterns of diploid and triploid hybrids, fluorescence chromosome location had been carried out, maternal 5S gene (M-386) were used as the probe. The triploid hybrids contained fewer fluorescence loci numbers than the maternal species. The results indicated that participation of paternal 5S gene in the triploid hybrid genome had degraded the match rates of M-386 probe. Conclusions: Our study is the first to investigate the cellular formation processes of natural allopolyploids in hybrid fish, the cellular polyploidization process may be caused by failure formation of the second polarized genome during the meiosis, and our results will provide the molecular basis of hybrid vigor in interspecies hybridization.
Department[Huang, Wen; Li, Shuisheng; Zhang, Yong; Lin, Haoran] Sun Yat Sen Univ, Inst Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China; [Huang, Wen; Li, Shuisheng; Zhang, Yong; Lin, Haoran] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China; [Huang, Wen; Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 510301, Guangdong, Peoples R China; [Huang, Wen; Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Qinbo; Wang, Yude; Liu, Shaojun] Hunan Normal Univ, Coll Life Sci, State Educ Minist China, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China; [Yang, Huirong] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China; [Hu, Chaoqun; Zhang, Yong; Lin, Haoran] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China ; LMB
KeywordNatural Polyploidization Embryo Development Grouper 5s Gene Heterosis
Subject AreaGenetics & Heredity
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15277
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorZhang, Y (reprint author), Sun Yat Sen Univ, Inst Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China.; Zhang, Y (reprint author), Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China.; Liu, SJ (reprint author), Hunan Normal Univ, Coll Life Sci, State Educ Minist China, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China.
Recommended Citation
GB/T 7714
Huang, Wen,Qin, Qinbo,Yang, Huirong,et al. Formation of diploid and triploid hybrid groupers (hybridization of Epinephelus coioides female x Epinephelus lanceolatus male) and their 5S gene analysis[J]. BMC GENETICS,2016,17:-.
APA Huang, Wen.,Qin, Qinbo.,Yang, Huirong.,Li, Shuisheng.,Hu, Chaoqun.,...&Liu, SJ .(2016).Formation of diploid and triploid hybrid groupers (hybridization of Epinephelus coioides female x Epinephelus lanceolatus male) and their 5S gene analysis.BMC GENETICS,17,-.
MLA Huang, Wen,et al."Formation of diploid and triploid hybrid groupers (hybridization of Epinephelus coioides female x Epinephelus lanceolatus male) and their 5S gene analysis".BMC GENETICS 17(2016):-.
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