Exploring fungal diversity in deep-sea sediments from Okinawa Trough using high-throughput Illumina sequencing
Zhang, Xiao-Yong; Wang, Guang-Hua; Xu, Xin-Ya; Nong, Xu-Hua; Wang, Jie; Amin, Muhammad; Qi, Shu-Hua; Qi, SH (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS
卷号116页码:99-105
摘要The present study investigated the fungal diversity in four different deep-sea sediments from Okinawa Trough using high-throughput Illumina sequencing of the nuclear ribosomal internal transcribed spacer 1 (ITS1). A total of 40,297 fungal ITS1 sequences clustered into 420 operational taxonomic units (OTUs) with 97% sequence similarity and 170 taxa were recovered from these sediments. Most ITS1 sequences (78%) belonged to the phylum Ascomycota, followed by Basidiomycota (17.3%), Zygomycota (1.5%) and Chytridiomycota (0.8%), and a small proportion (2.4%) belonged to unassigned fungal phyla. Compared with previous studies on fungal diversity of sediments from deep-sea environments by culture-dependent approach and clone library analysis, the present result suggested that Illumina sequencing had been dramatically accelerating the discovery of fungal community of deep-sea sediments. Furthermore, our results revealed that Sordariomycetes was the most diverse and abundant fungal class in this study, challenging the traditional view that the diversity of Sordariomycetes phylotypes was low in the deepsea environments. In addition, more than 12 taxa accounted for 21.5% sequences were found to be rarely reported as deep-sea fungi, suggesting the deep-sea sediments from Okinawa Trough harbored a plethora of different fungal communities compared with other deep-sea environments. To our knowledge, this study is the first exploration of the fungal diversity in deep-sea sediments from Okinawa Trough using high-throughput Illumina sequencing. (C) 2016 Elsevier Ltd. All rights reserved.
部门归属[Zhang, Xiao-Yong; Wang, Guang-Hua; Xu, Xin-Ya; Nong, Xu-Hua; Wang, Jie; Amin, Muhammad; Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Xiao-Yong; Wang, Guang-Hua; Xu, Xin-Ya; Nong, Xu-Hua; Wang, Jie; Amin, Muhammad; Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Xiao-Yong; Wang, Guang-Hua; Xu, Xin-Ya; Nong, Xu-Hua; Wang, Jie; Amin, Muhammad; Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China ; LMB
关键词Fungal Diversity Deep-sea Sediments Okinawa Trough Illumina Sequencing Its1
学科领域Oceanography
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15283
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Qi, SH (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
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Zhang, Xiao-Yong,Wang, Guang-Hua,Xu, Xin-Ya,et al. Exploring fungal diversity in deep-sea sediments from Okinawa Trough using high-throughput Illumina sequencing[J]. DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS,2016,116:99-105.
APA Zhang, Xiao-Yong.,Wang, Guang-Hua.,Xu, Xin-Ya.,Nong, Xu-Hua.,Wang, Jie.,...&Qi, SH .(2016).Exploring fungal diversity in deep-sea sediments from Okinawa Trough using high-throughput Illumina sequencing.DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS,116,99-105.
MLA Zhang, Xiao-Yong,et al."Exploring fungal diversity in deep-sea sediments from Okinawa Trough using high-throughput Illumina sequencing".DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS 116(2016):99-105.
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