MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper
Guo, Minglan; Wei, Jingguang; Zhou, Yongcan; Qin, Qiwei; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Zhou, YC (reprint author), Hainan Univ, Coll Marine Sci, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Peoples R China.
2016
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号57页码:419-427
摘要Mitogen-activated protein kinase 7 (MKK7) is one of the major stress-activated protein kinase (SAPK)activating kinases in response to environmental or physiological stimuli. Here a MKK7 named as Ec-MKK7 was identified from orange-spotted grouper, Epinephelus coioides. The full-length cDNA of Ec-MKK7 was 1853 bp, with an open reading frame (ORF) of 1272 bp encoding a putative protein of 423 amino acids. A characteristic S-K-A-K-T motif was contained in the domain of dual-specificity protein kinase, mitogen-activated protein kinase kinase 7 (PKc_MKK7). Intracellular localization showed that Ec-MKK7 was localized in both the cytoplasm and the nucleus of grouper spleen (GS) and/or grouper brain (EAGB) cells. Moreover, Ec-MKK7 was universally expressed in all examined tissues and showed expression modulation to challenges of lipopolysacchride (LPS), Singapore grouper iridovirus (SGIV) and polyriboinosinic polyribocytidylic acid (poly I:C) in vivo. A gene targeting strategy over-expressing Ec-MKK7 was performed to examine the activities of MKK7 to viral infection in vitro. Our data showed that Ec-MKK7 was involved in the evasion and replication of SGIV but played an antiviral role to the infection of nervous necrosis virus (NNV). All results demonstrated that Ec-MKK7 could play important roles in grouper innate immunity and show distinct functions on virus infection. (C) 2016 ElSevier Ltd. All rights reserved.
部门归属[Guo, Minglan; Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510301, Guangdong, Peoples R China; [Guo, Minglan; Wei, Jingguang] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Zhou, Yongcan] Hainan Univ, Coll Marine Sci, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Peoples R China ; LMB
关键词Epinephelus Coioides Mkk7 Molecular Characterization Singapore Grouper Iridovirus (Sgiv) Nervous Necrosis Virus (Nnv)
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15287
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Zhou, YC (reprint author), Hainan Univ, Coll Marine Sci, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Peoples R China.
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Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,et al. MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper[J]. FISH & SHELLFISH IMMUNOLOGY,2016,57:419-427.
APA Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,Qin, Qiwei,Qin, QW ,&Zhou, YC .(2016).MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper.FISH & SHELLFISH IMMUNOLOGY,57,419-427.
MLA Guo, Minglan,et al."MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper".FISH & SHELLFISH IMMUNOLOGY 57(2016):419-427.
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