The RNA Chaperone Hfq Is Involved in Colony Morphology, Nutrient Utilization and Oxidative and Envelope Stress Response in Vibrio alginolyticus
Deng, Yiqin; Chen, Chang; Zhao, Zhe; Zhao, Jingjing; Jacq, Annick; Huang, Xiaochun; Yang, Yiying; Chen, C (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China.; Chen, C (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Guangdong, Peoples R China.; Chen, C (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Xisha Nansha Ocean Observat & Res Stn, Guangzhou, Guangdong, Peoples R China.
2016
发表期刊PLOS ONE
卷号11期号:9页码:e0163689
摘要Hfq is a global regulator that is involved in environmental adaptation of bacteria and in pathogenicity. To gain insight into the role of Hfq in Vibrio alginolyticus, an hfq deletion mutant was constructed in V. alginolyticus ZJ-T strain and phenotypically characterized. Deletion of hfq led to an alteration of colony morphology and reduced extracellular polysaccharide production, a general impairment of growth in both rich medium and minimal media with different carbon sources or amino acids, enhanced sensitivity to oxidative stress and to several antibiotics. Furthermore, a differential transcriptomic analysis showed significant changes of transcript abundance for 306 protein coding genes, with 179 genes being up regulated and 127 down-regulated. Several of these changes could be related to the observed phenotypes of the mutant. Transcriptomic data also provided evidence for the induction of the extracytoplasmic stress response in absence of Hfq. Altogether, these findings point to broad regulatory functions for Hfq in V. alginolyticus cells, likely to underlie an important role in pathogenicity.
部门归属[Deng, Yiqin; Chen, Chang; Zhao, Zhe; Zhao, Jingjing; Huang, Xiaochun; Yang, Yiying] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China; [Deng, Yiqin; Chen, Chang; Zhao, Zhe; Zhao, Jingjing; Huang, Xiaochun; Yang, Yiying] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Guangdong, Peoples R China; [Chen, Chang] Chinese Acad Sci, South China Sea Inst Oceanol, Xisha Nansha Ocean Observat & Res Stn, Guangzhou, Guangdong, Peoples R China; [Deng, Yiqin; Huang, Xiaochun; Yang, Yiying] Univ Chinese Acad Sci, Beijing, Peoples R China; [Jacq, Annick] Univ Paris Saclay, Univ Paris Sud, CNRS, CEA,Inst Integrat Biol Cell I2BC, Gif Sur Yvette, France ; LMB
学科领域Science & Technology - Other Topics
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15290
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Chen, C (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China.; Chen, C (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Guangdong, Peoples R China.; Chen, C (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Xisha Nansha Ocean Observat & Res Stn, Guangzhou, Guangdong, Peoples R China.
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Deng, Yiqin,Chen, Chang,Zhao, Zhe,et al. The RNA Chaperone Hfq Is Involved in Colony Morphology, Nutrient Utilization and Oxidative and Envelope Stress Response in Vibrio alginolyticus[J]. PLOS ONE,2016,11(9):e0163689.
APA Deng, Yiqin.,Chen, Chang.,Zhao, Zhe.,Zhao, Jingjing.,Jacq, Annick.,...&Chen, C .(2016).The RNA Chaperone Hfq Is Involved in Colony Morphology, Nutrient Utilization and Oxidative and Envelope Stress Response in Vibrio alginolyticus.PLOS ONE,11(9),e0163689.
MLA Deng, Yiqin,et al."The RNA Chaperone Hfq Is Involved in Colony Morphology, Nutrient Utilization and Oxidative and Envelope Stress Response in Vibrio alginolyticus".PLOS ONE 11.9(2016):e0163689.
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