Development and characterization of aptamer-based enzyme-linked apta-sorbent assay for the detection of Singapore grouper iridovirus infection
Li, P.; Zhou, L.; Wei, J.; Yu, Y.; Yang, M.; Wei, S.; Qin, Q.; Wei, S; Qin, Q (reprint author), Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol, 164 W Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊JOURNAL OF APPLIED MICROBIOLOGY
卷号121期号:3页码:634-643
摘要AimsSingapore grouper iridovirus (SGIV) is a devastating aquaculture virus responsible for heavy economic losses to grouper, Epinephelus sp. aquaculture. The aim of this study was to develop a rapid and sensitive detection method for SGIV infections in infected groupers. Methods and ResultsWe previously generated DNA aptamers against SGIV-infected cells. In this study, we established and characterized a novel aptamer (Q3)-based enzyme-linked apta-sorbent assay (ELASA) for the detection of SGIV infection in Epinephelus coioides. The Q3-based ELASA could detect SGIV infection rapidly invitro and invivo, with high specificity and stability. Q3-based ELASA specifically recognized SGIV-infected cells, but not other-virus-infected cells or uninfected cells. Q3-based ELASA detected SGIV infection in a dose-dependent manner at Q3 concentrations as low as 125nmol l(-1). The results in relation to SGIV-infected cells (5x10(4)), incubation time (1min) and incubation temperature (37 degrees C) demonstrated that Q3-based ELASA could detect SGIV infection quickly and stably, superior to antibody-based enzyme-linked immunosorbent assay. Q3-based ELASA could detect the presence of SGIV infection in kidney, liver and spleen samples invivo, at dilutions of 1/50, 1/100 and 1/50 respectively. The complete detection process took 1-2h. ConclusionsQ3-based ELASA could be a useful tool for diagnosing SGIV infection. Significance and Impact of the StudyThis is the first developed aptamer-based ELASA for detecting SGIV infection, and is widely applicable in grouper aquaculture industry in light of its rapidity, and high specificity and stability.
部门归属[Li, P.; Zhou, L.; Wei, J.; Yu, Y.; Yang, M.; Wei, S.; Qin, Q.] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol, 164 W Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Li, P.; Zhou, L.; Yu, Y.] Univ Chinese Acad Sci, Beijing, Peoples R China; [Qin, Q.] South China Agr Univ, Coll Marine Sci, Guangzhou, Guangdong, Peoples R China ; LMB
关键词Aptamer-based Elasa Grouper Sgiv Infection Specificity Stability
学科领域Biotechnology & Applied Microbiology ; Microbiology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15296
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Wei, S; Qin, Q (reprint author), Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol, 164 W Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
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Li, P.,Zhou, L.,Wei, J.,et al. Development and characterization of aptamer-based enzyme-linked apta-sorbent assay for the detection of Singapore grouper iridovirus infection[J]. JOURNAL OF APPLIED MICROBIOLOGY,2016,121(3):634-643.
APA Li, P..,Zhou, L..,Wei, J..,Yu, Y..,Yang, M..,...&Qin, Q .(2016).Development and characterization of aptamer-based enzyme-linked apta-sorbent assay for the detection of Singapore grouper iridovirus infection.JOURNAL OF APPLIED MICROBIOLOGY,121(3),634-643.
MLA Li, P.,et al."Development and characterization of aptamer-based enzyme-linked apta-sorbent assay for the detection of Singapore grouper iridovirus infection".JOURNAL OF APPLIED MICROBIOLOGY 121.3(2016):634-643.
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