Negative regulation of the antiviral response by grouper LGP2 against fish viruses
Yu, Yepin; Huang, Youhua; Yang, Ying; Wang, Shaowen; Yang, Min; Huang, Xiaohong; Qin, Qiwei; Huang, XH; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号56页码:358-366
摘要Laboratory of genetics and physiology 2 (LGP2), a member of RIG-I like receptor (RLR) family, plays crucial roles in modulating cellular antiviral response during viral infection. However, the detailed roles of LGP2 in different virus infection were controversial up to now. Here, we cloned a LGP2 gene from orange-spotted grouper (EcLGP2) and investigated its roles in response to grouper virus infection. EcLGP2 encoded a 678-aa protein which shared 83% identity to sea perch (Lateolabrax japonicas). Amino acid alignment showed that EcLGP2 contained three conserved domains, including a DEAD/DEAH box helicase domain, a helicase superfamily C-terminal domain and a C-terminal domain of RIG-I. In healthy grouper, the transcript of EcLGP2 could be predominantly detected in kidney, gill, fin, spleen and skin. Subcellular localization analysis showed that EcLGP2 distributed throughout the cytoplasm in grouper cells. Notably, the intracellular distribution of EcLGP2 was altered at the late stage of Singapore grouper iridovirus (SGIV) infection, but remained unchanged during red-spotted grouper nervous necrosis virus (RGNNV) infection. Moreover, overexpression of EcLGP2 in vitro significantly enhanced the viral replication of SGIV and RGNNV, evidenced by the acceleration of CPE occurrence and the up-regulation of the viral gene transcription or protein synthesis. Further studies indicated that overexpression of EcLGP2 decreased the expression level of interferon related molecules or effectors, including IRF3, IRF7, ISG15, IFP35, MXI, MXII, and MDA5, suggesting that the negative feedback of interferon immune response by EcLGP2 might contribute to the enhancement of RGNNV infection. Moreover, the expression levels of pro-inflammation cytokines, including IL-8 and TNF alpha were significantly decreased, but that of IL-6 was increased by the ectopic expression of EcLGP2. Thus, our results will contribute greatly to understanding the roles of fish LGP2 in innate immune response during iridovirus and nodavirus infection. (C) 2016 Elsevier Ltd. All rights reserved.
部门归属[Yu, Yepin; Huang, Youhua; Yang, Ying; Wang, Shaowen; Yang, Min; Huang, Xiaohong; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Yu, Yepin; Huang, Youhua; Yang, Ying; Wang, Shaowen; Yang, Min; Huang, Xiaohong; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Yu, Yepin; Huang, Youhua; Yang, Ying; Wang, Shaowen; Yang, Min; Huang, Xiaohong; Qin, Qiwei] Univ Chinese Acad Sci, Beijing, Peoples R China; [Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China ; LMB
关键词Lgp2 Grouper Iridovirus Rgnnv Interferon
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15299
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Huang, XH; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
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GB/T 7714
Yu, Yepin,Huang, Youhua,Yang, Ying,et al. Negative regulation of the antiviral response by grouper LGP2 against fish viruses[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:358-366.
APA Yu, Yepin.,Huang, Youhua.,Yang, Ying.,Wang, Shaowen.,Yang, Min.,...&Qin, QW .(2016).Negative regulation of the antiviral response by grouper LGP2 against fish viruses.FISH & SHELLFISH IMMUNOLOGY,56,358-366.
MLA Yu, Yepin,et al."Negative regulation of the antiviral response by grouper LGP2 against fish viruses".FISH & SHELLFISH IMMUNOLOGY 56(2016):358-366.
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