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Tetraodon nigroviridis: A model of Vibrio parahaemolyticus infection
Peng, Wan; Shi, Yu; Li, Gao-Fei; He, Liang-Ge; Liang, Yao-Si; Zhang, Yong; Zhou, Li-Bin; Lin, Hao-Ran; Lu, Dan-Qi; Lu, DQ (reprint author), Sun Yat Sen Univ, State Key Lab Biocontrol, Inst Aquat Econ Anim, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China.; Lu, DQ (reprint author), Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China.
2016
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume56Pages:388-396
AbstractVibriosis is the most common bacterial diseases and brings great economic loss on aquaculture. Vibrio parahaemolyticus (V. parahaemolyticus), a gram-negative bacterium, has been identified as one main pathogens of Vibriosis. The pathogenic mechanism of V. parahaemolyticus is not entirely clear now. In our study, a model of V. parahaemolyticus infection of green-spotted puffer fish (Tetraodon nigroviridis) was established. T. nigroviridis were injected intraperitoneally (i.p.) with 200 mu L of V. parahaemolyticus (8 x 10(10) CFU/mL). V parahaemolyticus infection caused 64% mortality and infected some organs of T. nigroviridis. Histopathology studies revealed V. parahaemolyticus infection induced tissue structural changes, including adipose hollow space in the liver. Immunohistochemistry showed V. parahaemolyticus were present in infected tissue such as liver, head kidney and spleen. In livers of T nigroviridis infected by V. parahaemolyticus, the alkaline phosphatases (ALP) activity first gradually increased and then backed to normal level, a trend that was on the contrary to the expression profile of the miR-29b. Quantitative real-time PCR analysis showed that the expression level of TLR1, TLR2, TLR5, TLR9, TLR21, NOD1, NOD2 and IL-6 in response to V. parahaemolyticus infection decreased compared to that of non-infected fish. The establishment of the T. nigroviridis model of V. parahaemolyticus infection further confirmed V. parahaemolyticus spreads through the blood circulation system primary as an extracellular pathogen. Meanwhile, liver is an important target organ when infected by V. parahaemolyticus. miR-29b in liver was involved in the progress of liver steatosis during V. parahaemolyticus infection. Moreover, V. parahaemolyticus infection in vivo may have an effect of immunosuppression on host. (C) 2016 Elsevier Ltd. All rights reserved.
Department[Peng, Wan; Li, Gao-Fei; He, Liang-Ge; Liang, Yao-Si; Zhang, Yong; Lin, Hao-Ran; Lu, Dan-Qi] Sun Yat Sen Univ, State Key Lab Biocontrol, Inst Aquat Econ Anim, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China; [Peng, Wan; Li, Gao-Fei; He, Liang-Ge; Liang, Yao-Si; Zhang, Yong; Lin, Hao-Ran; Lu, Dan-Qi] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China; [Shi, Yu] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Zhou, Li-Bin] Huizhou Univ, Dept Life Sci, Huizhou 516007, Peoples R China ; LMB
KeywordVibrio Parahaemolyticus Tetraodon Nigroviridis Liver Mir-29b Pattern Recognition Receptors
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15300
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorLu, DQ (reprint author), Sun Yat Sen Univ, State Key Lab Biocontrol, Inst Aquat Econ Anim, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China.; Lu, DQ (reprint author), Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Peng, Wan,Shi, Yu,Li, Gao-Fei,et al. Tetraodon nigroviridis: A model of Vibrio parahaemolyticus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:388-396.
APA Peng, Wan.,Shi, Yu.,Li, Gao-Fei.,He, Liang-Ge.,Liang, Yao-Si.,...&Lu, DQ .(2016).Tetraodon nigroviridis: A model of Vibrio parahaemolyticus infection.FISH & SHELLFISH IMMUNOLOGY,56,388-396.
MLA Peng, Wan,et al."Tetraodon nigroviridis: A model of Vibrio parahaemolyticus infection".FISH & SHELLFISH IMMUNOLOGY 56(2016):388-396.
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