Functional Characterization of a Novel Marine Microbial Esterase and its Utilization in the Enantioselective Preparation of (R)-Methyl 2-Chloropropionate
Cao, Yingying; Deng, Dun; Sun, Aijun; Zhang, Yun; Hu, Yunfeng; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
2016
发表期刊APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷号180期号:2页码:210-227
摘要Chiral 2-chloropropanoic acids and their ester derivatives are crucial intermediates in the synthesis of many chemicals, especially herbicides. The enzymatic synthesis of chiral 2-chloropropanoic acids and their ester derivatives by esterases was not easily achieved, because the structural difference between the two enantiomers was too small to be recognized by esterases. Herein, we report the expression and functional characterization of one novel low temperature-resistant esterase EST12-7 identified from the genome of Pseudonocardia antitumoralis SCSIO 01299 isolated from the sediments of the South China Sea. Biocatalyst EST12-7 could hydrolyze racemic methyl 2-chloropropinate and generate optically pure (R)-methyl 2-chloropropinate with high enantiomeric excess (> 99 %) and conversion (> 49 %) after process optimization. Notably, the addition of different surfactants and using surfactants of different concentrations in the kinetic resolution catalyzed by EST12-7 could greatly affect the enantiomeric excess and conversion rate of product (R)-methyl 2-chloropropinate.
部门归属[Cao, Yingying; Deng, Dun; Sun, Aijun; Zhang, Yun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Cao, Yingying; Deng, Dun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Cao, Yingying; Deng, Dun; Sun, Aijun; Zhang, Yun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Hu, Yunfeng] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China ; LMB
关键词Novel Esterase Marine Microorganism Kinetic Resolution (r)-methyl 2-chloropropinate High Enantioselectivity
学科领域Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15301
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
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Cao, Yingying,Deng, Dun,Sun, Aijun,et al. Functional Characterization of a Novel Marine Microbial Esterase and its Utilization in the Enantioselective Preparation of (R)-Methyl 2-Chloropropionate[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,180(2):210-227.
APA Cao, Yingying.,Deng, Dun.,Sun, Aijun.,Zhang, Yun.,Hu, Yunfeng.,...&Hu, YF .(2016).Functional Characterization of a Novel Marine Microbial Esterase and its Utilization in the Enantioselective Preparation of (R)-Methyl 2-Chloropropionate.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,180(2),210-227.
MLA Cao, Yingying,et al."Functional Characterization of a Novel Marine Microbial Esterase and its Utilization in the Enantioselective Preparation of (R)-Methyl 2-Chloropropionate".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 180.2(2016):210-227.
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