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Functional Characterization of a Novel Dactylosporangium Esterase and Its Utilization in the Asymmetric Synthesis of (R)-Methyl Mandelate
Deng, Dun; Zhang, Yun; Sun, Aijun; Hu, Yunfeng; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
2016
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume180Issue:2Pages:228-247
AbstractOne novel esterase DAEst6 was identified from the genome of Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085. DAEst6 was further characterized to be an esterase which exhibited high resistance to high pH values. Esterase DAEst6 could resolve racemic methyl mandelate and generate (R)-methyl mandelate, one key drug intermediate, with an enantiomeric excess and a conversion of 99 and 49 %, respectively, after process optimization. The optimal working condition for the preparation of (R)-methyl mandelate through DAEst6 was found to be 10-mM racemic methyl mandelate, no organic co-solvents, pH 7.5, and 40 A degrees C, for 5 h. Our work was the first report about the functional characterization of one novel Dactylosporangium esterase and the utilization of one Dactylosporangium esterase in kinetic resolution. Dactylosporangium esterases represented by DAEst6 possess great potential in the generation of valuable chiral drug intermediates and chemicals.
Department[Deng, Dun; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Deng, Dun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Deng, Dun; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Hu, Yunfeng] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China ; LMB
KeywordNovel Esterase Dactylosporangium (r)-methyl Mandelate Kinetic Resolution High Enantiomeric Excess
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15302
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorHu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Deng, Dun,Zhang, Yun,Sun, Aijun,et al. Functional Characterization of a Novel Dactylosporangium Esterase and Its Utilization in the Asymmetric Synthesis of (R)-Methyl Mandelate[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,180(2):228-247.
APA Deng, Dun.,Zhang, Yun.,Sun, Aijun.,Hu, Yunfeng.,Hu, YF .,...&Hu, YF .(2016).Functional Characterization of a Novel Dactylosporangium Esterase and Its Utilization in the Asymmetric Synthesis of (R)-Methyl Mandelate.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,180(2),228-247.
MLA Deng, Dun,et al."Functional Characterization of a Novel Dactylosporangium Esterase and Its Utilization in the Asymmetric Synthesis of (R)-Methyl Mandelate".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 180.2(2016):228-247.
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