Functional Characterization of a Novel Dactylosporangium Esterase and Its Utilization in the Asymmetric Synthesis of (R)-Methyl Mandelate
Deng, Dun; Zhang, Yun; Sun, Aijun; Hu, Yunfeng; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
2016
发表期刊APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷号180期号:2页码:228-247
摘要One novel esterase DAEst6 was identified from the genome of Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085. DAEst6 was further characterized to be an esterase which exhibited high resistance to high pH values. Esterase DAEst6 could resolve racemic methyl mandelate and generate (R)-methyl mandelate, one key drug intermediate, with an enantiomeric excess and a conversion of 99 and 49 %, respectively, after process optimization. The optimal working condition for the preparation of (R)-methyl mandelate through DAEst6 was found to be 10-mM racemic methyl mandelate, no organic co-solvents, pH 7.5, and 40 A degrees C, for 5 h. Our work was the first report about the functional characterization of one novel Dactylosporangium esterase and the utilization of one Dactylosporangium esterase in kinetic resolution. Dactylosporangium esterases represented by DAEst6 possess great potential in the generation of valuable chiral drug intermediates and chemicals.
部门归属[Deng, Dun; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Deng, Dun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Deng, Dun; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Hu, Yunfeng] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China ; LMB
关键词Novel Esterase Dactylosporangium (r)-methyl Mandelate Kinetic Resolution High Enantiomeric Excess
学科领域Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15302
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
推荐引用方式
GB/T 7714
Deng, Dun,Zhang, Yun,Sun, Aijun,et al. Functional Characterization of a Novel Dactylosporangium Esterase and Its Utilization in the Asymmetric Synthesis of (R)-Methyl Mandelate[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,180(2):228-247.
APA Deng, Dun.,Zhang, Yun.,Sun, Aijun.,Hu, Yunfeng.,Hu, YF .,...&Hu, YF .(2016).Functional Characterization of a Novel Dactylosporangium Esterase and Its Utilization in the Asymmetric Synthesis of (R)-Methyl Mandelate.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,180(2),228-247.
MLA Deng, Dun,et al."Functional Characterization of a Novel Dactylosporangium Esterase and Its Utilization in the Asymmetric Synthesis of (R)-Methyl Mandelate".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 180.2(2016):228-247.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Functional Character(1292KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。