MHC class II alpha polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides
Yang, Min; Wei, Jingguang; Li, Pengfei; Wei, Shina; Huang, Youhua; Qin, Qiwei; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊AQUACULTURE
卷号462页码:2016-10-16
摘要The major histocompatibility complex (MHC) is a crucial component of the immune systems of vertebrate animals and is associated with their resistance/susceptibility to pathogenic diseases. In this study, the association between MHC II alpha gene polymorphisms and disease resistance/susceptibility was analyzed in the orange-spotted grouper (Epinephelus coioides), by challenging it with Singapore grouper iridovirus (SGIV). Thirty-seven alleles showing high levels of polymorphism (24.7% mutation sites) were isolated from 80 individuals. In the peptide-binding region, the ratio of nonsynonymous (dN) substitutions to synonymous (dS) substitutions was 1.427 (>1), suggesting that the loci are evolving under positive balancing selection. Eight alleles were selected to estimate their distributions in high-resistance (HR) and high-susceptibility (HS) groups of fish. The EPCO-DAA*0101 allelewas significantly associated with susceptibility to SGIV infection (P < 0.05) and the EPCO-DAA*0104, EPCO-DAA* 0601, EPCO-DAA*1101, and EPCO-DAA*1201 alleles were significantly associated with resistance to SGIV (P < 0.05 for each). To confirm these correlations, an additional independent challenge experiment was performed in the Wenchang population of the orange-spotted grouper. The frequency distribution showed that the EPCO-DAA*0101 allele was significantly more frequent in the Wenchang HS (WC-HS) group than in the WC-HR group (P < 0.05), whereas the EPCO-DAA*1101 allele was significantly more frequent in the WC-HR group than in the WC-HS group (P < 0.05), consistent with the first challenge. However, the frequency distributions of the EPCO-DAA*0104, EPCO-DAA*0601, and EPCO-DAA*1201 alleles did not differ significantly between the WC-HR and WC-HS groups (P > 0.05). These results indicate that the EPCO-DAA*0101 allele confers susceptibility to SGIV infection, whereas the EPCO-DAA*1101 allele confers resistance to it. These disease-resistance- related MHC markers could be useful for the molecular-marker-assisted selective breeding of the orange-spotted grouper. (C) 2016 Elsevier B.V. All rights reserved.
部门归属[Yang, Min; Wei, Jingguang; Li, Pengfei; Wei, Shina; Huang, Youhua; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510641, Guangdong, Peoples R China; [Li, Pengfei] Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China ; LMB
关键词Disease Resistance Major Histocompatibility Complex Ii Alpha Orange-spotted Grouper Selective Breeding Program Singapore Grouper Iridovirus
学科领域Fisheries ; Marine & Freshwater Biology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15304
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
推荐引用方式
GB/T 7714
Yang, Min,Wei, Jingguang,Li, Pengfei,et al. MHC class II alpha polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides[J]. AQUACULTURE,2016,462:2016-10-16.
APA Yang, Min.,Wei, Jingguang.,Li, Pengfei.,Wei, Shina.,Huang, Youhua.,...&Qin, QW .(2016).MHC class II alpha polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides.AQUACULTURE,462,2016-10-16.
MLA Yang, Min,et al."MHC class II alpha polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides".AQUACULTURE 462(2016):2016-10-16.
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