SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Characterization of a novel marine microbial esterase and its use to make D-methyl lactate
Wang, Yilong; Zhang, Yun; Sun, Aijun; Hu, Yunfeng; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Sout China Sea Bioresource Exploitat & Utilizat C, Guangzhou 510275, Guangdong, Peoples R China.
2016
Source PublicationCHINESE JOURNAL OF CATALYSIS
Volume37Issue:8Pages:1396-1402
AbstractA novel marine microbial esterase PHE14 was cloned from the genome of Pseudomonas oryzihabitans HUP022 isolated from the deep sea of the western Pacific Ocean. Esterase PHE14 exhibited very good tolerance to most organic solvents, surfactants and metal ions tested, thus making it a good esterase candidate for organic synthesis that requires an organic solvent, surfactants or metal ions. Esterase PHE14 was utilized as a biocatalyst in the asymmetric synthesis of D-methyl lactate by enzymatic kinetic resolution. D-methyl lactate is a key chiral chemical. Contrary to some previous reports, the addition of an organic solvent and surfactants in the enzymatic reaction did not have a beneficial effect on the kinetic resolution catalyzed by esterase PHE14. Our study is the first report on the preparation of the enantiomerically enriched product D-methyl lactate by enzymatic kinetic resolution. The desired enantiomerically enriched product D-methyl lactate was obtained with a high enantiomeric excess of 99% and yield of 88.7% after process optimization. The deep sea microbial esterase PHE14 is a green biocatalyst with very good potential in asymmetric synthesis in industry and can replace the traditional organic synthesis that causes pollution to the environment. (C) 2016, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
Department[Wang, Yilong; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Yilong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Hu, Yunfeng] Sout China Sea Bioresource Exploitat & Utilizat C, Guangzhou 510275, Guangdong, Peoples R China ; LMB
KeywordEsterase Marine Microorganism Biocatalysis Kinetic Resolution D-methyl Lactate Hydrolysis
Subject AreaChemistry ; Engineering
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15308
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorHu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Sout China Sea Bioresource Exploitat & Utilizat C, Guangzhou 510275, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Wang, Yilong,Zhang, Yun,Sun, Aijun,et al. Characterization of a novel marine microbial esterase and its use to make D-methyl lactate[J]. CHINESE JOURNAL OF CATALYSIS,2016,37(8):1396-1402.
APA Wang, Yilong.,Zhang, Yun.,Sun, Aijun.,Hu, Yunfeng.,Hu, YF .,...&Hu, YF .(2016).Characterization of a novel marine microbial esterase and its use to make D-methyl lactate.CHINESE JOURNAL OF CATALYSIS,37(8),1396-1402.
MLA Wang, Yilong,et al."Characterization of a novel marine microbial esterase and its use to make D-methyl lactate".CHINESE JOURNAL OF CATALYSIS 37.8(2016):1396-1402.
Files in This Item:
File Name/Size DocType Version Access License
Characterization of (590KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.