Characterization of a novel marine microbial esterase and its use to make D-methyl lactate
Wang, Yilong; Zhang, Yun; Sun, Aijun; Hu, Yunfeng; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Sout China Sea Bioresource Exploitat & Utilizat C, Guangzhou 510275, Guangdong, Peoples R China.
2016
发表期刊CHINESE JOURNAL OF CATALYSIS
卷号37期号:8页码:1396-1402
摘要A novel marine microbial esterase PHE14 was cloned from the genome of Pseudomonas oryzihabitans HUP022 isolated from the deep sea of the western Pacific Ocean. Esterase PHE14 exhibited very good tolerance to most organic solvents, surfactants and metal ions tested, thus making it a good esterase candidate for organic synthesis that requires an organic solvent, surfactants or metal ions. Esterase PHE14 was utilized as a biocatalyst in the asymmetric synthesis of D-methyl lactate by enzymatic kinetic resolution. D-methyl lactate is a key chiral chemical. Contrary to some previous reports, the addition of an organic solvent and surfactants in the enzymatic reaction did not have a beneficial effect on the kinetic resolution catalyzed by esterase PHE14. Our study is the first report on the preparation of the enantiomerically enriched product D-methyl lactate by enzymatic kinetic resolution. The desired enantiomerically enriched product D-methyl lactate was obtained with a high enantiomeric excess of 99% and yield of 88.7% after process optimization. The deep sea microbial esterase PHE14 is a green biocatalyst with very good potential in asymmetric synthesis in industry and can replace the traditional organic synthesis that causes pollution to the environment. (C) 2016, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
部门归属[Wang, Yilong; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Yilong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Hu, Yunfeng] Sout China Sea Bioresource Exploitat & Utilizat C, Guangzhou 510275, Guangdong, Peoples R China ; LMB
关键词Esterase Marine Microorganism Biocatalysis Kinetic Resolution D-methyl Lactate Hydrolysis
学科领域Chemistry ; Engineering
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15308
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Sout China Sea Bioresource Exploitat & Utilizat C, Guangzhou 510275, Guangdong, Peoples R China.
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Wang, Yilong,Zhang, Yun,Sun, Aijun,et al. Characterization of a novel marine microbial esterase and its use to make D-methyl lactate[J]. CHINESE JOURNAL OF CATALYSIS,2016,37(8):1396-1402.
APA Wang, Yilong.,Zhang, Yun.,Sun, Aijun.,Hu, Yunfeng.,Hu, YF .,...&Hu, YF .(2016).Characterization of a novel marine microbial esterase and its use to make D-methyl lactate.CHINESE JOURNAL OF CATALYSIS,37(8),1396-1402.
MLA Wang, Yilong,et al."Characterization of a novel marine microbial esterase and its use to make D-methyl lactate".CHINESE JOURNAL OF CATALYSIS 37.8(2016):1396-1402.
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