Grouper TRIM13 exerts negative regulation of antiviral immune response against nodavirus
Huang, Youhua; Yang, Min; Yu, Yepin; Yang, Ying; Zhou, Linli; Huang, Xiaohong; Qin, Qiwei; Huang, XH; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号55页码:106-115
摘要The tripartite motif (TRIM)-containing proteins have attracted particular attention to their multiple functions in different biological processes. TRIM13, a member of the TRIM family, is a RING domain containing E3 ubiquitin ligase which plays critical roles in diverse cellular processes including cell death, cancer and antiviral immunity. In this study, a TRIM13 homolog from orange spotted grouper, Epinephelus coioides (EcTRIM13) was cloned and characterized. The full-length of EcTRIM13 cDNA encoded a polypeptide of 399 amino acids which shared 81% identity with TRIM13 homolog from large yellow croaker (Larimichthys crocea). Amino acid alignment analysis showed that EcTRIM13 contained conserved RING finger and B-box domain. Expression patterns analysis indicated that EcTRIM13 was abundant in liver, spleen, kidney, intestine and gill. Moreover, the transcript of EcTRIM13 in grouper spleen was differently regulated after injection with Singapore grouper iridovirus (SGIV) or polyinosin-polycytidylic acid (poly I:C). Under fluorescence microscopy, we observed the tubular structure in wild type EcTRIM13 transfected cells, but the RING domain mutant resulted in the fluorescence distribution was changed and the bright punctate fluorescence was evenly situated throughout the cytoplasm, suggesting that the RING domain was essential for its accurate localization. Overexpression of EcTRIM13 in vitro obviously increased the replication of red spotted grouper nervous necrosis virus (RGNNV), and the enhancing effect of EcTRIM13 on virus replication was affected by the RING domain. Furthermore, the ectopic expression of EcTRIM13 not only negatively regulated the interferon promoter activity induced by interferon regulator factor (IRF) 3, IRF7, and melanoma differentiation-associated protein 5 (MDA5), but also decreased the expression of several interferon related factors. In addition, the overexpression of EcTRIM13 also differently regulated the transcription of pro-inflammatory factors. Together, our results firstly demonstrated that fish TRIM13 exerted negative regulation of antiviral response against nodavirus infection. (C) 2016 Elsevier Ltd. All rights reserved.
部门归属[Huang, Youhua; Yang, Min; Yu, Yepin; Yang, Ying; Zhou, Linli; Huang, Xiaohong; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Yu, Yepin; Yang, Ying; Zhou, Linli; Huang, Xiaohong; Qin, Qiwei] Univ Chinese Acad Sci, Beijing, Peoples R China ; LMB
关键词Trim13 Grouper Rgnnv Antiviral Interferon
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15309
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Huang, XH; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
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Huang, Youhua,Yang, Min,Yu, Yepin,et al. Grouper TRIM13 exerts negative regulation of antiviral immune response against nodavirus[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:106-115.
APA Huang, Youhua.,Yang, Min.,Yu, Yepin.,Yang, Ying.,Zhou, Linli.,...&Qin, QW .(2016).Grouper TRIM13 exerts negative regulation of antiviral immune response against nodavirus.FISH & SHELLFISH IMMUNOLOGY,55,106-115.
MLA Huang, Youhua,et al."Grouper TRIM13 exerts negative regulation of antiviral immune response against nodavirus".FISH & SHELLFISH IMMUNOLOGY 55(2016):106-115.
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