Cloning, expression and characterization of a lipase gene from marine bacterium Pseudoalteromonas lipolytica SCSIO 04301
Su, Hongfei; Mai, Zhimao; Zhang, Si; Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bio Resources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊JOURNAL OF OCEAN UNIVERSITY OF CHINA
卷号15期号:6页码:1051-1058
摘要A lipase gene, lip1233, isolated from Pseudoalteromonas lipolytica SCSIO 04301, was cloned and expressed in E. coli. The enzyme comprised 810 amino acid residues with a deduced molecular weight of 80 kDa. Lip1233 was grouped into the lipase family X because it contained a highly conserved motif GHSLG. The recombinant enzyme was purified with Ni-NTA affinity chromatography. The optimal temperature and pH value of Lip1233 were 45A degrees C and 8.0, respectively. It retained more than 70% of original activity after being incubated in pH ranging from 6.0 to 9.5 for 30 min. It was stable when the temperature was below 45A degrees C, but was unstable when the temperature was above 55A degrees C. Most metal ions tested had no significant effect on the activity of Lip1233. Lip1233 remained more than original activity in some organic solvents at the concentration of 30% (v/v). It retained more than 30% activity after incubated in pure organic solvents for 12 h, while in hexane the activity was nearly 100%. Additionally, Lip1233 exhibited typical halotolerant characteristic as it was active under 4M NaCl. Lip1233 powder could catalyze efficiently the synthesis of fructose esters in hexane at 40A degrees C. These characteristics demonstrated that Lip1233 is applicable to elaborate food processing and organic synthesis.
部门归属[Su, Hongfei; Mai, Zhimao; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bio Resources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Su, Hongfei; Mai, Zhimao; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Su, Hongfei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; 广东省海洋药物重点实验室
关键词Lipase Organic-solvent-tolerance Halotolerance Fructose Ester Pseudoalteromonas Lipolytica Scsio 04301
学科领域Oceanography
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15334
专题广东省应用海洋生物学重点实验室
通讯作者Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bio Resources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.
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Su, Hongfei,Mai, Zhimao,Zhang, Si,et al. Cloning, expression and characterization of a lipase gene from marine bacterium Pseudoalteromonas lipolytica SCSIO 04301[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2016,15(6):1051-1058.
APA Su, Hongfei,Mai, Zhimao,Zhang, Si,Zhang, S ,&Zhang, S .(2016).Cloning, expression and characterization of a lipase gene from marine bacterium Pseudoalteromonas lipolytica SCSIO 04301.JOURNAL OF OCEAN UNIVERSITY OF CHINA,15(6),1051-1058.
MLA Su, Hongfei,et al."Cloning, expression and characterization of a lipase gene from marine bacterium Pseudoalteromonas lipolytica SCSIO 04301".JOURNAL OF OCEAN UNIVERSITY OF CHINA 15.6(2016):1051-1058.
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