Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate
Liang Jiayuan; Sun Aijun; Zhang Yun; Deng Dun; Wang Yongfei; Ma Sanmei; Hu Yunfeng; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China.
2016
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号34期号:6页码:1269-1277
摘要Genomic mining has identified a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21 (DE3) and further functionally characterized. Under optimal conditions (10 mmol/L substrate, pH 6.0, 2 h at 40 A degrees C), this esterase can hydrolyze racemic methyl mandelate to (R)-methyl mandelate with very high optical purity (e.e. > 99%) and yield (nearly 90%). Interestingly, the stereoselectivity of this esterase is opposite to that of two previously reported lipases that can generate (S)-methyl mandelate through the hydrolysis of racemic methyl mandelate. No organic solvents or other additives were required to optimize the optical purity and production of the final chiral product (R)-methyl mandelate, which can potentially simplify the production procedure of (R)-methyl mandelate catalyzed by esterase.
部门归属[Liang Jiayuan; Sun Aijun; Zhang Yun; Deng Dun; Hu Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Liang Jiayuan; Sun Aijun; Zhang Yun; Deng Dun; Hu Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Hu Yunfeng] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China; [Wang Yongfei; Ma Sanmei] Jinan Univ, Dept Biotechnol, Guangzhou 510632, Guangdong, Peoples R China ; 广东省海洋药物重点实验室
关键词Alkaline Esterase Kinetic Resolution (r)-methyl Mandelate High Optical Purity Opposite Stereoselectivity
学科领域Marine & Freshwater Biology ; Oceanography
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15336
专题广东省应用海洋生物学重点实验室
通讯作者Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China.
推荐引用方式
GB/T 7714
Liang Jiayuan,Sun Aijun,Zhang Yun,et al. Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(6):1269-1277.
APA Liang Jiayuan.,Sun Aijun.,Zhang Yun.,Deng Dun.,Wang Yongfei.,...&Hu, YF .(2016).Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(6),1269-1277.
MLA Liang Jiayuan,et al."Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.6(2016):1269-1277.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Functional character(361KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。