Isolation and Characterization of a Glycosyl Hydrolase Family 16 beta-Agarase from a Mangrove Soil Metagenomic Library
Mai, Zhimao; Su, Hongfei; Zhang, Si; Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号17期号:8页码:1360
摘要A mangrove soil metagenomic library was constructed and a beta-agarase gene designated as AgaML was isolated by functional screening. The gene encoded for a 659-amino-acids polypeptide with an estimated molecular mass of 71.6 kDa. The deduced polypeptide sequences of AgaML showed the highest identity of 73% with the glycoside hydrolase family 16 beta-agarase from Microbulbifer agarilyticus in the GenBank database. AgaML was cloned and highly expressed in Escherichia coli BL21(DE3). The purified recombinant protein, AgaML, showed optimal activity at 50 degrees C and pH 7.0. The kinetic parameters of K-m and V-max values toward agarose were 4.6 mg.mL(-1) and 967.5 mu M.min(-1).mg(-1), respectively. AgaML hydrolyzed the beta-1,4-glycosidic linkages of agar to generate neoagarotetraose (NA4) and neoagarohexaose (NA6) as the main products. These characteristics suggest that AgaML has potential application in cosmetic, pharmaceuticals and food industries.
部门归属[Mai, Zhimao; Su, Hongfei; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; 广东省海洋药物重点实验室
关键词Agarase Metagenomic Library Agar Neoagaro-oligosaccharides
学科领域Biochemistry & Molecular Biology ; Chemistry
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15337
专题广东省应用海洋生物学重点实验室
通讯作者Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
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Mai, Zhimao,Su, Hongfei,Zhang, Si,et al. Isolation and Characterization of a Glycosyl Hydrolase Family 16 beta-Agarase from a Mangrove Soil Metagenomic Library[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(8):1360.
APA Mai, Zhimao,Su, Hongfei,Zhang, Si,&Zhang, S .(2016).Isolation and Characterization of a Glycosyl Hydrolase Family 16 beta-Agarase from a Mangrove Soil Metagenomic Library.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(8),1360.
MLA Mai, Zhimao,et al."Isolation and Characterization of a Glycosyl Hydrolase Family 16 beta-Agarase from a Mangrove Soil Metagenomic Library".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.8(2016):1360.
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