De Novo Transcriptome Analysis of Two Seahorse Species (Hippocampus erectus and H. mohnikei) and the Development of Molecular Markers for Population Genetics
Lin, Qiang; Luo, Wei; Wan, Shiming; Gao, Zexia; Lin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510275, Guangdong, Peoples R China.
2016
发表期刊PLOS ONE
卷号11期号:4页码:e0154096
摘要Seahorse conservation has been performed utilizing various strategies for many decades, and the deeper understanding of genomic information is necessary to more efficiently protect the germplasm resources of seahorse species. However, little genetic information about seahorses currently exists in the public databases. In this study, high-throughput RNA sequencing for two seahorse species, Hippocampus erectus and H. mohnikei, was carried out, and de novo assembly generated 37,506 unigenes for H. erectus and 36,113 unigenes for H. mohnikei. Among them, 17,338 (46.23%) unigenes for H. erectus and 17,900 (49.57%) for H. mohnikei were successfully annotated based on the information available from the public databases. Through comparing the unigenes of two seahorse species, 7,802 candidate orthologous genes were identified and 5,268 genes among them could be annotated. In addition, gene ontology analysis of two species was similarly performed on biological processes, cellular components, and molecular functions. Twenty-four and twenty-one unigenes in H. erectus and H. mohnikei were annotated in the biosynthesis of unsaturated fatty acids pathways, and both seahorses lacked the Delta 12 and Delta 15 desaturases. Total of 8,992 and 9,116 SSR loci were obtained from H. erectus and H. mohnikei unigenes, respectively. Dozens of SSR were developed and then applied to assess the population genetic diversity, as well as cross-amplified in a related species, H. trimaculatus. The H-O and H-E values of the tested populations for H. erectus, H. mohnikei, and H. trimaculatus were medium. These resources would facilitate the conservation of the species through a better understanding of the genomics and comparative genome analysis within the Hippocampus genus.
部门归属[Lin, Qiang; Luo, Wei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510275, Guangdong, Peoples R China; [Wan, Shiming; Gao, Zexia] Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Key Lab Agr Anim Genet Breeding & Reprod,Coll Fis, Minist Educ,Key Lab Freshwater Anim Breeding,Mini, Wuhan 430070, Peoples R China ; LTO
学科领域Science & Technology - Other Topics
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15373
专题热带海洋环境国家重点实验室(LTO)
通讯作者Lin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510275, Guangdong, Peoples R China.
推荐引用方式
GB/T 7714
Lin, Qiang,Luo, Wei,Wan, Shiming,et al. De Novo Transcriptome Analysis of Two Seahorse Species (Hippocampus erectus and H. mohnikei) and the Development of Molecular Markers for Population Genetics[J]. PLOS ONE,2016,11(4):e0154096.
APA Lin, Qiang,Luo, Wei,Wan, Shiming,Gao, Zexia,&Lin, Q .(2016).De Novo Transcriptome Analysis of Two Seahorse Species (Hippocampus erectus and H. mohnikei) and the Development of Molecular Markers for Population Genetics.PLOS ONE,11(4),e0154096.
MLA Lin, Qiang,et al."De Novo Transcriptome Analysis of Two Seahorse Species (Hippocampus erectus and H. mohnikei) and the Development of Molecular Markers for Population Genetics".PLOS ONE 11.4(2016):e0154096.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
De Novo Transcriptom(2135KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。