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Characterization of the Deep-Sea Streptomyces sp SCSIO 02999 Derived VapC/VapB Toxin-Antitoxin System in Escherichia coli
Guo, Yunxue; Yao, Jianyun; Sun, Chenglong; Wen, Zhongling; Wang, Xiaoxue; Guo, YX; Wang, XX (reprint author), Chinese Acad Sci, Res Network Appl Microbiol RNAM Ctr Marine Microb, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationTOXINS
Volume8Issue:7Pages:195
AbstractToxin-antitoxin (TA) systems are small genetic elements that are ubiquitous in prokaryotes. Most studies on TA systems have focused on commensal and pathogenic bacteria; yet very few studies have focused on TAs in marine bacteria, especially those isolated from a deep sea environment. Here, we characterized a type II VapC/VapB TA system from the deep-sea derived Streptomyces sp. SCSIO 02999. The VapC (virulence-associated protein) protein belongs to the PIN (PilT N-terminal) superfamily. Overproduction of VapC strongly inhibited cell growth and resulted in a bleb-containing morphology in E. coli. The toxicity of VapC was neutralized through direct protein-protein interaction by a small protein antitoxin VapB encoded by a neighboring gene. Antitoxin VapB alone or the VapB/VapC complex negatively regulated the vapBC promoter activity. We further revealed that three conserved Asp residues in the PIN domain were essential for the toxic effect of VapC. Additionally, the VapC/VapB TA system stabilized plasmid in E. coli. Furthermore, VapC cross-activated transcription of several TA operons via a partially Lon-dependent mechanism in E. coli, and the activated toxins accumulated more preferentially than their antitoxin partners. Collectively, we identified and characterized a new deep sea TA system in the deep sea Streptomyces sp. and demonstrated that the VapC toxin in this system can cross-activate TA operons in E. coli.
Department[Guo, Yunxue; Yao, Jianyun; Sun, Chenglong; Wen, Zhongling; Wang, Xiaoxue] Chinese Acad Sci, Res Network Appl Microbiol RNAM Ctr Marine Microb, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China; [Yao, Jianyun; Sun, Chenglong; Wen, Zhongling] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LMB
KeywordToxin-antitoxin Vapc/vapb Deep Sea Streptomyces
Subject AreaToxicology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15385
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorGuo, YX; Wang, XX (reprint author), Chinese Acad Sci, Res Network Appl Microbiol RNAM Ctr Marine Microb, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Guo, Yunxue,Yao, Jianyun,Sun, Chenglong,et al. Characterization of the Deep-Sea Streptomyces sp SCSIO 02999 Derived VapC/VapB Toxin-Antitoxin System in Escherichia coli[J]. TOXINS,2016,8(7):195.
APA Guo, Yunxue.,Yao, Jianyun.,Sun, Chenglong.,Wen, Zhongling.,Wang, Xiaoxue.,...&Wang, XX .(2016).Characterization of the Deep-Sea Streptomyces sp SCSIO 02999 Derived VapC/VapB Toxin-Antitoxin System in Escherichia coli.TOXINS,8(7),195.
MLA Guo, Yunxue,et al."Characterization of the Deep-Sea Streptomyces sp SCSIO 02999 Derived VapC/VapB Toxin-Antitoxin System in Escherichia coli".TOXINS 8.7(2016):195.
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