Characterization of the Deep-Sea Streptomyces sp SCSIO 02999 Derived VapC/VapB Toxin-Antitoxin System in Escherichia coli
Guo, Yunxue; Yao, Jianyun; Sun, Chenglong; Wen, Zhongling; Wang, Xiaoxue; Guo, YX; Wang, XX (reprint author), Chinese Acad Sci, Res Network Appl Microbiol RNAM Ctr Marine Microb, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊TOXINS
卷号8期号:7页码:195
摘要Toxin-antitoxin (TA) systems are small genetic elements that are ubiquitous in prokaryotes. Most studies on TA systems have focused on commensal and pathogenic bacteria; yet very few studies have focused on TAs in marine bacteria, especially those isolated from a deep sea environment. Here, we characterized a type II VapC/VapB TA system from the deep-sea derived Streptomyces sp. SCSIO 02999. The VapC (virulence-associated protein) protein belongs to the PIN (PilT N-terminal) superfamily. Overproduction of VapC strongly inhibited cell growth and resulted in a bleb-containing morphology in E. coli. The toxicity of VapC was neutralized through direct protein-protein interaction by a small protein antitoxin VapB encoded by a neighboring gene. Antitoxin VapB alone or the VapB/VapC complex negatively regulated the vapBC promoter activity. We further revealed that three conserved Asp residues in the PIN domain were essential for the toxic effect of VapC. Additionally, the VapC/VapB TA system stabilized plasmid in E. coli. Furthermore, VapC cross-activated transcription of several TA operons via a partially Lon-dependent mechanism in E. coli, and the activated toxins accumulated more preferentially than their antitoxin partners. Collectively, we identified and characterized a new deep sea TA system in the deep sea Streptomyces sp. and demonstrated that the VapC toxin in this system can cross-activate TA operons in E. coli.
部门归属[Guo, Yunxue; Yao, Jianyun; Sun, Chenglong; Wen, Zhongling; Wang, Xiaoxue] Chinese Acad Sci, Res Network Appl Microbiol RNAM Ctr Marine Microb, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China; [Yao, Jianyun; Sun, Chenglong; Wen, Zhongling] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LMB
关键词Toxin-antitoxin Vapc/vapb Deep Sea Streptomyces
学科领域Toxicology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15385
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Guo, YX; Wang, XX (reprint author), Chinese Acad Sci, Res Network Appl Microbiol RNAM Ctr Marine Microb, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China.
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Guo, Yunxue,Yao, Jianyun,Sun, Chenglong,et al. Characterization of the Deep-Sea Streptomyces sp SCSIO 02999 Derived VapC/VapB Toxin-Antitoxin System in Escherichia coli[J]. TOXINS,2016,8(7):195.
APA Guo, Yunxue.,Yao, Jianyun.,Sun, Chenglong.,Wen, Zhongling.,Wang, Xiaoxue.,...&Wang, XX .(2016).Characterization of the Deep-Sea Streptomyces sp SCSIO 02999 Derived VapC/VapB Toxin-Antitoxin System in Escherichia coli.TOXINS,8(7),195.
MLA Guo, Yunxue,et al."Characterization of the Deep-Sea Streptomyces sp SCSIO 02999 Derived VapC/VapB Toxin-Antitoxin System in Escherichia coli".TOXINS 8.7(2016):195.
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