SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Antiviral function of grouper MDA5 against iridovirus and nodavirus
Huang, Youhua; Yu, Yepin; Yang, Ying; Yang, Min; Zhou, Linli; Huang, Xiaohong; Qin, Qiwei; Huang, XH; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume54Pages:188-196
AbstractMelanoma differentiation-associated gene 5 (MDA5) is a critical member of retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family which can recognize viral RNA and enhances antiviral response in host cells. In this study, a MDA5 homolog from orange spotted grouper (Epinephelus coioides) (EcMDA5) was cloned, and its roles on grouper virus infection were characterized. The full-length EcMDA5 cDNA encoded a polypeptide of 982 amino acids with 74% identity with MDA5 homolog from rock bream (Oplegnathus fasciatus). Amino acid alignment analysis indicated that EcMDA5 contained three functional domains: two caspase activation and recruitment domain (CARDs), a DEAD box helicase-like (DExDc) domain, a helicase superfamily C-terminal domain (HELICc), and a C-terminal regulatory domain (RD). Upon challenge with Singapore grouper iridovirus (SGIV) or polyinosin-polycytidylic acid (poly I:C), the transcript of EcMDA5 was significantly up-regulated especially at the early stage post-injection. Under fluorescence microscopy, we observed that EcMDA5 mostly localized in the cytoplasm of grouper spleen (GS) cells. Interestingly, during virus infection, the distribution pattern of EcMDA5 was significantly altered in SGIV infected cells, but not in red spotted grouper nervous necrosis virus (RGNNV) infected cells, suggested that EcMDA5 might interact with viral proteins during SGIV infection. The ectopic expression of EcMDA5 in vitro obviously delayed virus infection induced cytopathic effect (CPE) progression and significantly inhibited viral gene transcription of RGNNV and SGIV. Moreover, over expression of EcMDA5 not only significantly increased interferon (IFN) and IFN-stimulated response element (ISRE) promoter activities in a dose dependent manner, but also enhanced the expression of IRF3, IRF7 and TRAF6. In addition, the transcription level of the proinflammatory factors, including TNF-alpha, IL-6 and IL-8 were differently altered by EcMDA5 overexpression during SGIV or RGNNV infection, suggesting that the regulation on proinflammatory cytokines by EcMDA5 were also important for RGNNV infection. Together, our results demonstrated for the first time that the inhibitory effect of fish MDA5 on iridovirus replication might be mainly through the regulation of proinflammatory cytokines. (C) 2016 Elsevier Ltd. All rights reserved.
Department[Huang, Youhua; Yu, Yepin; Yang, Ying; Yang, Min; Zhou, Linli; Huang, Xiaohong; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Huang, Youhua; Yu, Yepin; Yang, Ying; Yang, Min; Zhou, Linli; Huang, Xiaohong; Qin, Qiwei] Univ Chinese Acad Sci, Beijing, Peoples R China ; LMB
KeywordMda5 Grouper Iridovirus Rgnnv Antiviral
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15387
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorHuang, XH; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Huang, Youhua,Yu, Yepin,Yang, Ying,et al. Antiviral function of grouper MDA5 against iridovirus and nodavirus[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:188-196.
APA Huang, Youhua.,Yu, Yepin.,Yang, Ying.,Yang, Min.,Zhou, Linli.,...&Qin, QW .(2016).Antiviral function of grouper MDA5 against iridovirus and nodavirus.FISH & SHELLFISH IMMUNOLOGY,54,188-196.
MLA Huang, Youhua,et al."Antiviral function of grouper MDA5 against iridovirus and nodavirus".FISH & SHELLFISH IMMUNOLOGY 54(2016):188-196.
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