Molecular cloning and characterization of a galectin-1 homolog in orange-spotted grouper, Epinephelus coioides
Chen, Xiuli; Wei, Jingguang; Xu, Meng; Yang, Min; Li, Pingfei; Wei, Shina; Huang, Youhua; Qin, Qiwei; Wei, JG; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.; Qin, QW (reprint author), South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China.; Wei, JG (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号54页码:333-341
摘要As a member of animal lectin family, galectin has the functions of pathogen recognition, anti-bacteria and anti-virus. In the present study, a galectin-1 homolog (EcGel-1) from grouper (Epinephelus coioides) was cloned and its possible role in fish immunity was analyzed. The full length cDNA of EcGel-1 is 504 bp, including a 408 bp open reading frame (ORF) which encodes 135 amino acids with a molecular mass of 15.19 kDa. Quantitative real-time PCR analysis indicated that EcGel-1 was constitutively expressed in all analyzed tissues of healthy grouper. The expression of EcGel-1 in the spleen of grouper was differentially up -regulated challenged with Singapore grouper iridovirus (SGIV), poly (I:C), and LPS. EcGel-1 was abundantly distributed in the cytoplasm in GS cells. Recombinant EcGel-1(rEcGel-1) protein can make chicken erythrocyte aggregation, and combine with gram negative bacteria and gram positive bacteria in the presence of 2-Mercaptoethanol (beta-ME). Taken together, the results showed that EcGel-1 may be an important molecule involved in pathogen recognition and pathogen elimination in the innate immunity of grouper. (C) 2016 Elsevier Ltd. All rights reserved.
部门归属[Chen, Xiuli; Wei, Jingguang; Yang, Min; Li, Pingfei; Wei, Shina; Huang, Youhua; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Xiuli; Li, Pingfei; Qin, Qiwei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Xiuli; Wei, Jingguang; Yang, Min; Li, Pingfei; Wei, Shina; Huang, Youhua; Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Chen, Xiuli; Wei, Jingguang; Yang, Min; Li, Pingfei; Wei, Shina; Huang, Youhua] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Xu, Meng] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China ; LMB
关键词Epinephelus Coioides Galectin-1 Molecular Cloning Sgiv
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15389
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Wei, JG; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.; Qin, QW (reprint author), South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China.; Wei, JG (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China.
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Chen, Xiuli,Wei, Jingguang,Xu, Meng,et al. Molecular cloning and characterization of a galectin-1 homolog in orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:333-341.
APA Chen, Xiuli.,Wei, Jingguang.,Xu, Meng.,Yang, Min.,Li, Pingfei.,...&Wei, JG .(2016).Molecular cloning and characterization of a galectin-1 homolog in orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,54,333-341.
MLA Chen, Xiuli,et al."Molecular cloning and characterization of a galectin-1 homolog in orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 54(2016):333-341.
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