Knowledge Management System Of South China Sea Institute of Oceanology,CAS
| Molecular cloning and characterization of a galectin-1 homolog in orange-spotted grouper, Epinephelus coioides | |
| Chen, Xiuli; Wei, Jingguang; Xu, Meng; Yang, Min; Li, Pingfei; Wei, Shina; Huang, Youhua; Qin, Qiwei; Wei, JG; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.; Qin, QW (reprint author), South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China.; Wei, JG (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China. | |
| 2016 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| Volume | 54Pages:333-341 |
| Abstract | As a member of animal lectin family, galectin has the functions of pathogen recognition, anti-bacteria and anti-virus. In the present study, a galectin-1 homolog (EcGel-1) from grouper (Epinephelus coioides) was cloned and its possible role in fish immunity was analyzed. The full length cDNA of EcGel-1 is 504 bp, including a 408 bp open reading frame (ORF) which encodes 135 amino acids with a molecular mass of 15.19 kDa. Quantitative real-time PCR analysis indicated that EcGel-1 was constitutively expressed in all analyzed tissues of healthy grouper. The expression of EcGel-1 in the spleen of grouper was differentially up -regulated challenged with Singapore grouper iridovirus (SGIV), poly (I:C), and LPS. EcGel-1 was abundantly distributed in the cytoplasm in GS cells. Recombinant EcGel-1(rEcGel-1) protein can make chicken erythrocyte aggregation, and combine with gram negative bacteria and gram positive bacteria in the presence of 2-Mercaptoethanol (beta-ME). Taken together, the results showed that EcGel-1 may be an important molecule involved in pathogen recognition and pathogen elimination in the innate immunity of grouper. (C) 2016 Elsevier Ltd. All rights reserved. |
| Department | [Chen, Xiuli; Wei, Jingguang; Yang, Min; Li, Pingfei; Wei, Shina; Huang, Youhua; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Xiuli; Li, Pingfei; Qin, Qiwei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Xiuli; Wei, Jingguang; Yang, Min; Li, Pingfei; Wei, Shina; Huang, Youhua; Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Chen, Xiuli; Wei, Jingguang; Yang, Min; Li, Pingfei; Wei, Shina; Huang, Youhua] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Xu, Meng] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China ; LMB |
| Keyword | Epinephelus Coioides Galectin-1 Molecular Cloning Sgiv |
| Subject Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| Document Type | 期刊论文 |
| Identifier | http://ir.scsio.ac.cn/handle/344004/15389 |
| Collection | 中科院海洋生物资源可持续利用重点实验室 |
| Corresponding Author | Wei, JG; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.; Qin, QW (reprint author), South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China.; Wei, JG (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China. |
| Recommended Citation GB/T 7714 | Chen, Xiuli,Wei, Jingguang,Xu, Meng,et al. Molecular cloning and characterization of a galectin-1 homolog in orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:333-341. |
| APA | Chen, Xiuli.,Wei, Jingguang.,Xu, Meng.,Yang, Min.,Li, Pingfei.,...&Wei, JG .(2016).Molecular cloning and characterization of a galectin-1 homolog in orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,54,333-341. |
| MLA | Chen, Xiuli,et al."Molecular cloning and characterization of a galectin-1 homolog in orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 54(2016):333-341. |
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