Establishment of a new cell line from the snout tissue of golden pompano Trachinotus ovatus, and its application in virus susceptibility
Yu, Y.; Wei, S.; Wang, Z.; Huang, X.; Huang, Y.; Cai, J.; Li, C.; Qin, Q.; Qin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Qin, Q (reprint author), South China Agr Univ, Coll Marine Sci, Guangzhou 510641, Guangdong, Peoples R China.
2016
发表期刊JOURNAL OF FISH BIOLOGY
卷号88期号:6页码:2251-2262
摘要A new marine-fish cell line, designated GPS, was established from the snout tissue of golden pompano Trachinotus ovatus. GPS cells multiplied well in Leibovitz's L-15 containing 10% foetal bovine serum (FBS) at 28 degrees C and the cells have been subcultured for >60 passages. Polymerase chain reaction (PCR) amplification of 16S ribosomal (r) RNA confirmed the origin of this cell line from T. ovatus. Chromosome analysis showed that GPS cells exhibited chromosomal aneuploidy with a modal chromosome number of 54. Bright green fluorescence signal was observed in enhanced green fluorescent protein (EGFP)-N3 transfected cells, indicating that GPS cells could be used to investigate gene functions in vitro. The GPS cells were highly susceptible to Singapore grouper iridovirus (SGIV), which was demonstrated by the presence of severe cytopathic effect (CPE) and increased viral titres. Real-time quantitative PCR and Western blot analysis showed that the viral gene transcription and protein synthesis occurred during SGIV infection in GPS cells. Thus, this study described the characteristic of a new cell line from the snout tissue of T. ovatus that could be used as a tool for propagation of iridovirus and genetic manipulation to investigate host-pathogen interactions. (C) 2016 The Fisheries Society of the British Isles
部门归属[Yu, Y.; Wei, S.; Huang, X.; Huang, Y.; Qin, Q.] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Yu, Y.; Wei, S.; Huang, X.; Huang, Y.] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Qin, Q.] South China Agr Univ, Coll Marine Sci, Guangzhou 510641, Guangdong, Peoples R China; [Wang, Z.; Li, C.] Qinghai Univ, Coll Ecol Environm Engn, Xining 810016, Peoples R China; [Cai, J.] Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China ; LMB
关键词Cell Line Snout Transgenic Manipulation Virus Infection
学科领域Fisheries ; Marine & Freshwater Biology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15398
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Qin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.; Qin, Q (reprint author), South China Agr Univ, Coll Marine Sci, Guangzhou 510641, Guangdong, Peoples R China.
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GB/T 7714
Yu, Y.,Wei, S.,Wang, Z.,et al. Establishment of a new cell line from the snout tissue of golden pompano Trachinotus ovatus, and its application in virus susceptibility[J]. JOURNAL OF FISH BIOLOGY,2016,88(6):2251-2262.
APA Yu, Y..,Wei, S..,Wang, Z..,Huang, X..,Huang, Y..,...&Qin, Q .(2016).Establishment of a new cell line from the snout tissue of golden pompano Trachinotus ovatus, and its application in virus susceptibility.JOURNAL OF FISH BIOLOGY,88(6),2251-2262.
MLA Yu, Y.,et al."Establishment of a new cell line from the snout tissue of golden pompano Trachinotus ovatus, and its application in virus susceptibility".JOURNAL OF FISH BIOLOGY 88.6(2016):2251-2262.
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