Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control
Yan, Ai-Fen; Chen, Ting; Chen, Shuang; Ren, Chun-Hua; Hu, Chao-Qun; Cai, Yi-Ming; Liu, Fang; Tang, Dong-Sheng; Chen, T (reprint author), Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.; Chen, T (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China.
2016
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号17期号:6页码:783
摘要In mammals, leptin is a peripheral satiety factor that inhibits feeding by regulating a variety of appetite-related hormones in the brain. However, most of the previous studies examining leptin in fish feeding were performed with mammalian leptins, which share very low sequence homologies with fish leptins. To elucidate the function and mechanism of endogenous fish leptins in feeding regulation, recombinant goldfish leptin-AI and leptin-AII were expressed in methylotrophic yeast and purified by immobilized metal ion affinity chromatography (IMAC). By intraperitoneal (IP) injection, both leptin-AI and leptin-AII were shown to inhibit the feeding behavior and to reduce the food consumption of goldfish in 2 h. In addition, co-treatment of leptin-AI or leptin-AII could block the feeding behavior and reduce the food consumption induced by neuropeptide Y (NPY) injection. High levels of leptin receptor (lepR) mRNA were detected in the hypothalamus, telencephalon, optic tectum and cerebellum of the goldfish brain. The appetite inhibitory effects of leptins were mediated by downregulating the mRNA levels of orexigenic NPY, agouti-related peptide (AgRP) and orexin and upregulating the mRNA levels of anorexigenic cocaine-amphetamine-regulated transcript (CART), cholecystokinin (CCK), melanin-concentrating hormone (MCH) and proopiomelanocortin (POMC) in different areas of the goldfish brain. Our study, as a whole, provides new insights into the functions and mechanisms of leptins in appetite control in a fish model.
部门归属[Yan, Ai-Fen; Liu, Fang; Tang, Dong-Sheng] Foshan Univ, Coll Med, Foshan 528000, Peoples R China; [Chen, Ting; Ren, Chun-Hua; Hu, Chao-Qun; Cai, Yi-Ming] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Ting; Ren, Chun-Hua; Hu, Chao-Qun] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China; [Chen, Shuang] Univ Hong Kong, Li Ka Shing Fac Med, Sch Biomed Sci, Hong Kong, Hong Kong, Peoples R China ; LMB
关键词Leptin Goldfish Methylotrophic Yeast Feeding Control Gene Regulation Appetite Regulators
学科领域Biochemistry & Molecular Biology ; Chemistry
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15402
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Chen, T (reprint author), Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.; Chen, T (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China.
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GB/T 7714
Yan, Ai-Fen,Chen, Ting,Chen, Shuang,et al. Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(6):783.
APA Yan, Ai-Fen.,Chen, Ting.,Chen, Shuang.,Ren, Chun-Hua.,Hu, Chao-Qun.,...&Chen, T .(2016).Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(6),783.
MLA Yan, Ai-Fen,et al."Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.6(2016):783.
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