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Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control
Yan, Ai-Fen; Chen, Ting; Chen, Shuang; Ren, Chun-Hua; Hu, Chao-Qun; Cai, Yi-Ming; Liu, Fang; Tang, Dong-Sheng; Chen, T (reprint author), Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.; Chen, T (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China.
2016
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume17Issue:6Pages:783
AbstractIn mammals, leptin is a peripheral satiety factor that inhibits feeding by regulating a variety of appetite-related hormones in the brain. However, most of the previous studies examining leptin in fish feeding were performed with mammalian leptins, which share very low sequence homologies with fish leptins. To elucidate the function and mechanism of endogenous fish leptins in feeding regulation, recombinant goldfish leptin-AI and leptin-AII were expressed in methylotrophic yeast and purified by immobilized metal ion affinity chromatography (IMAC). By intraperitoneal (IP) injection, both leptin-AI and leptin-AII were shown to inhibit the feeding behavior and to reduce the food consumption of goldfish in 2 h. In addition, co-treatment of leptin-AI or leptin-AII could block the feeding behavior and reduce the food consumption induced by neuropeptide Y (NPY) injection. High levels of leptin receptor (lepR) mRNA were detected in the hypothalamus, telencephalon, optic tectum and cerebellum of the goldfish brain. The appetite inhibitory effects of leptins were mediated by downregulating the mRNA levels of orexigenic NPY, agouti-related peptide (AgRP) and orexin and upregulating the mRNA levels of anorexigenic cocaine-amphetamine-regulated transcript (CART), cholecystokinin (CCK), melanin-concentrating hormone (MCH) and proopiomelanocortin (POMC) in different areas of the goldfish brain. Our study, as a whole, provides new insights into the functions and mechanisms of leptins in appetite control in a fish model.
Department[Yan, Ai-Fen; Liu, Fang; Tang, Dong-Sheng] Foshan Univ, Coll Med, Foshan 528000, Peoples R China; [Chen, Ting; Ren, Chun-Hua; Hu, Chao-Qun; Cai, Yi-Ming] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Ting; Ren, Chun-Hua; Hu, Chao-Qun] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China; [Chen, Shuang] Univ Hong Kong, Li Ka Shing Fac Med, Sch Biomed Sci, Hong Kong, Hong Kong, Peoples R China ; LMB
KeywordLeptin Goldfish Methylotrophic Yeast Feeding Control Gene Regulation Appetite Regulators
Subject AreaBiochemistry & Molecular Biology ; Chemistry
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15402
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorChen, T (reprint author), Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.; Chen, T (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Yan, Ai-Fen,Chen, Ting,Chen, Shuang,et al. Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(6):783.
APA Yan, Ai-Fen.,Chen, Ting.,Chen, Shuang.,Ren, Chun-Hua.,Hu, Chao-Qun.,...&Chen, T .(2016).Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(6),783.
MLA Yan, Ai-Fen,et al."Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.6(2016):783.
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