Cloning, Expression, and Characterization of a Cold-Active and Organic Solvent-Tolerant Lipase from Aeromicrobium sp SCSIO 25071
Su, Hongfei; Mai, Zhimao; Yang, Jian; Xiao, Yunzhu; Tian, Xinpeng; Zhang, Si; Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷号26期号:6页码:1067-1076
摘要The gene encoding lipase (Lip98) from Aeromicrobium sp. SCSIO 25071 was cloned and functionally expressed in Escherichia coli. Lip98 amino acid sequence shares the highest (49%) identity to Rhodococcus jostii RHA1 lipase and contains a novel motif (GHSEG), which is different from other clusters in the lipase superfamily. The recombinant lipase was purified to homogeneity with Ni-NTA affinity chromatography. Lip98 showed an apparent molecular mass of 30 kDa on SDS gel. The optimal temperature and pH value for enzymatic activity were recorded at 30Q degrees C and 7.5, respectively. Lip98 exhibited high activity at low temperatures with 35% maximum activity at 0 degrees C and good stability at temperatures below 35 degrees C. Its calculated activation energy was 4.12 kcal/mol at the low temperature range of 15-30 degrees C. Its activity was slightly affected by some metal ions such as K+, Ca2+, and Na+. The activity of Lip98 was increased by various organic solvents such as DMSO, ethanol, acetone, and hexane with the concentration of 30% (v/v) and retained more than 30% residual activity in neat organic solvent. The unique characteristics of Lip98 imply that it is a promising candidate for industrial application as a nonaqueous biocatalyst and food additive.
部门归属[Su, Hongfei; Mai, Zhimao; Yang, Jian; Xiao, Yunzhu; Tian, Xinpeng; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Su, Hongfei; Xiao, Yunzhu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LMB
关键词Lipase Cold-active Solvent-tolerant Aeromicrobium Sp Scsio 25071
学科领域Biotechnology & Applied Microbiology ; Microbiology
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15403
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
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GB/T 7714
Su, Hongfei,Mai, Zhimao,Yang, Jian,et al. Cloning, Expression, and Characterization of a Cold-Active and Organic Solvent-Tolerant Lipase from Aeromicrobium sp SCSIO 25071[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2016,26(6):1067-1076.
APA Su, Hongfei.,Mai, Zhimao.,Yang, Jian.,Xiao, Yunzhu.,Tian, Xinpeng.,...&Zhang, S .(2016).Cloning, Expression, and Characterization of a Cold-Active and Organic Solvent-Tolerant Lipase from Aeromicrobium sp SCSIO 25071.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,26(6),1067-1076.
MLA Su, Hongfei,et al."Cloning, Expression, and Characterization of a Cold-Active and Organic Solvent-Tolerant Lipase from Aeromicrobium sp SCSIO 25071".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 26.6(2016):1067-1076.
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