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Population Relationship of Vibrio parahaemolyticus Isolates Derived from Aquaculture Ponds, a Seafood Market, Restaurants, and Clinical Samples
Gao, Lei; Deng, Yi Qin; Chen, Chang; Ke, Chang Wen; Li, Bo Sheng; Long, Yun Ying; Liu, Zhu Hong; Wei, Lu; Chen, C (reprint author), South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, 309B Bldg 1,164 Xingang Xi Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationFOODBORNE PATHOGENS AND DISEASE
Volume13Issue:6Pages:333-342
AbstractTo study the relationship between environmental and clinical populations of Vibrio parahaemolyticus, we collected in total 86 isolates from Southern China during one and a half years. Sixty-eight isolates were recovered from aquaculture ponds, a seafood market, and restaurants, and 18 isolates were recovered from clinical samples. Virulence gene analysis revealed that 25 isolates (14 clinical and 11 environmental) tested positive for tdh, but only 4 carried trh. Interestingly, none of the tdh(+) environmental isolates was recovered from ponds. Both environmental and clinical tdh(+) isolates, except for one clinical isolate, harbor type III secretion system 2 alpha (T3SS2 alpha) and T3SS2 beta-related genes, including vopB2 alpha, which was previously suggested to be absent from environmental strains. More than 70% of clinical isolates carried the pandemic marker of new toxRS (GS-PCR+), which was not present in the environmental isolates. Pulsed-field gel electrophoresis and multilocus sequence typing analysis showed a high degree of genetic diversity within the environmental isolates. In contrast, the clinical population formed a tight cluster that differed from the environmental isolates. These findings suggest that the pandemic strains of V. parahaemolyticus may not directly originate from marine animals. Rather the environments where they are maintained could serve as reservoirs for toxigenic, but not pandemic strains. These environments provide an ideal place for generation of new toxigenic strains through DNA exchange, which was revealed by extensive recombination events in recA sequences of the environmental isolates.
Department[Gao, Lei; Deng, Yi Qin; Chen, Chang; Long, Yun Ying; Liu, Zhu Hong; Wei, Lu] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China; [Ke, Chang Wen; Li, Bo Sheng] Guangdong Prov Ctr Dis Control & Prevent, Inst Pathogen Microorganisms, Guangzhou, Guangdong, Peoples R China ; LMB
Subject AreaFood Science & Technology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15410
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorChen, C (reprint author), South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, 309B Bldg 1,164 Xingang Xi Rd, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Gao, Lei,Deng, Yi Qin,Chen, Chang,et al. Population Relationship of Vibrio parahaemolyticus Isolates Derived from Aquaculture Ponds, a Seafood Market, Restaurants, and Clinical Samples[J]. FOODBORNE PATHOGENS AND DISEASE,2016,13(6):333-342.
APA Gao, Lei.,Deng, Yi Qin.,Chen, Chang.,Ke, Chang Wen.,Li, Bo Sheng.,...&Chen, C .(2016).Population Relationship of Vibrio parahaemolyticus Isolates Derived from Aquaculture Ponds, a Seafood Market, Restaurants, and Clinical Samples.FOODBORNE PATHOGENS AND DISEASE,13(6),333-342.
MLA Gao, Lei,et al."Population Relationship of Vibrio parahaemolyticus Isolates Derived from Aquaculture Ponds, a Seafood Market, Restaurants, and Clinical Samples".FOODBORNE PATHOGENS AND DISEASE 13.6(2016):333-342.
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