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PfSMAD4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster Pinctada fucata
Zhao, Mi; Shi, Yu; He, Maoxian; Huang, Xiande; Wang, Qi; He, MX (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationBMC DEVELOPMENTAL BIOLOGY
Volume16Pages:9
AbstractBackground: Mollusca is the second largest phylum in nature. The shell of molluscs is a remarkable example of a natural composite biomaterial. Biomineralization and how it affects mollusks is a popular research topic. The BMP-2 signaling pathway plays a canonical role in biomineralization. SMAD4 is an intracellular transmitter in the BMP signaling pathway in mammals, and some genomic data show SMAD4's involvment in BMP signaling in invertbrates, but whether SMAD4 plays a conservative role in pearl oyster, Pinctada fucata, still need to be tested. Results: In this study, we identified a SMAD4 gene (hereafter designated PfSMAD4) in pearl oyster Pinctada fucata. Bioinformatics analysis of PfSMAD4 showed high identity with its orthologs. PfSMAD4 was located in the cytoplasm in immunofluorescence assays and analyses of PfSMAD4 mRNA in tissues and developmental stages showed high expression in ovaries and D-shaped larvae. An RNA interference experiment, performed by PfSMAD4 double-stranded RNA (dsRNA) injection, demonstrated inhibition not only of nacre growth but also organic sheet formation with a decrease in PfSMAD4 expression. A knockdown experiment using PfBMP2 dsRNA showed decreased PfBMP2 and PfSMAD4 mRNA and irregular crystallization of the nacreous layer using scanning electron microscopy. In co-transfection experiments, PfBMP2-transactivated reporter constructs contained PfSMAD4 promoter sequences. Conclusions: Our results suggest that PfSMAD4 plays a role in biomineralization and can transduce BMP signals in P. fucata. Our data provides important clues about the molecular mechanisms that regulate biomineralization in pearl oyster.
Department[Zhao, Mi; Shi, Yu; He, Maoxian; Huang, Xiande; Wang, Qi] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Zhao, Mi; Wang, Qi] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LMB
KeywordSmad4 Biomineralization Bmp Signaling Pathway Pinctada Fucata
Subject AreaDevelopmental Biology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15462
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorHe, MX (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Zhao, Mi,Shi, Yu,He, Maoxian,et al. PfSMAD4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster Pinctada fucata[J]. BMC DEVELOPMENTAL BIOLOGY,2016,16:9.
APA Zhao, Mi,Shi, Yu,He, Maoxian,Huang, Xiande,Wang, Qi,&He, MX .(2016).PfSMAD4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster Pinctada fucata.BMC DEVELOPMENTAL BIOLOGY,16,9.
MLA Zhao, Mi,et al."PfSMAD4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster Pinctada fucata".BMC DEVELOPMENTAL BIOLOGY 16(2016):9.
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