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A thymosin beta-4 is involved in production of hemocytes and immune defense of Hong Kong oyster, Crassostrea hongkongensis
Li, Jun; Zhang, Yuehuan; Liu, Ying; Zhang, Yang; Xiang, Zhiming; Qu, Fufa; Yu, Ziniu; Zhang, Y; Yu, ZN (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, 164 W Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume57Pages:2016-01-09
AbstractThymosin beta-4 (T beta 4) is a ubiquitous protein with multiple and diverse intracellular and extracellular functions in vertebrates. In this study, the full-length cDNA of T beta 4 was cloned and identified in Crassostrea hongkongensis, designated as ChT beta 4. The full-length cDNA of ChT beta 4 consists of 530 bp with an open reading frame of 126 bp encoding a 41 amino acid polypeptide. SMART analysis indicated that there is one thymosin domain and a highly conserved actin-binding motif (18LKKTET23) in ChT beta 4. In vivo injection of recombinant ChT beta 4 protein could significantly increase total hemocytes count in oysters, and knockdown of the expression of ChT beta 4 resulted in a significant decrease in the circulating hemocytes. Tissue distribution analysis revealed a ubiquitous presence of ChT beta 4, with the highest expression in hemocytes. The upregulated transcripts of ChT beta 4 in response to bacterial challenge and tissue injury suggest that ChT beta 4 is involved in both innate immunity against pathogen infection and wound healing. Moreover, bacteria-clearance experiment showed ChT beta 4 could facilitate the clearance of injected bacteria in oysters. In vivo injection with ChT beta 4 resulted in reduction of the intracellular ROS in hemocytes, which was associated with increased expression of antioxidant enzymes Cu/Zn superoxide dismutase (SOD), Catalase, and Glutathione Peroxidase (GPX) by pre-treatment with ChT beta 4. These results suggest that ChT beta 4 is a thymosin beta-4 homolog and plays a vital role in the immune defense of C. hongkongensis. (C) 2015 Elsevier Ltd. All rights reserved.
Department[Li, Jun; Zhang, Yuehuan; Liu, Ying; Zhang, Yang; Xiang, Zhiming; Qu, Fufa; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Li, Jun; Zhang, Yuehuan; Liu, Ying; Zhang, Yang; Xiang, Zhiming; Qu, Fufa; Yu, Ziniu] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Peoples R China; [Liu, Ying] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China ; LMB
KeywordCrassostrea Hongkongensis Thymosin Beta-4 Total Hemocytes Count Antioxidant Enzymes
Subject AreaImmunology ; Zoology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15476
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorZhang, Y; Yu, ZN (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, 164 W Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Li, Jun,Zhang, Yuehuan,Liu, Ying,et al. A thymosin beta-4 is involved in production of hemocytes and immune defense of Hong Kong oyster, Crassostrea hongkongensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,57:2016-01-09.
APA Li, Jun.,Zhang, Yuehuan.,Liu, Ying.,Zhang, Yang.,Xiang, Zhiming.,...&Yu, ZN .(2016).A thymosin beta-4 is involved in production of hemocytes and immune defense of Hong Kong oyster, Crassostrea hongkongensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,57,2016-01-09.
MLA Li, Jun,et al."A thymosin beta-4 is involved in production of hemocytes and immune defense of Hong Kong oyster, Crassostrea hongkongensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 57(2016):2016-01-09.
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