Development of SNP markers in lined seahorse (Hippocampus erectus) based on transcriptome sequencing
Qu, Hongyue; Luo, Wei; Lin, Qiang; Lin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊CONSERVATION GENETICS RESOURCES
卷号8期号:1页码:2016-01-04
摘要The population of lined seahorse (Hippocampus erectus) rapidly reduced because of the heavy exploitation in the wild. The single nucleotide polymorphism (SNP), known as one of the popular third-generation molecular markers can be developed easier and faster from high-throughput transcriptome sequencing. In this study, we developed 46 novel SNP markers based on transcriptome of H. erectus. Genetic diversity analysis showed that observed heterozygosity (Ho) and expected heterozygosity (He) were varied from 0.1724 to 0.5250 and from 0.1588 to 0.5000, respectively, and the minor allele frequency value was from 0.0625 to 0.5000. Seven loci were significantly different from Hardy-Weinberg equilibrium (P < 0.05) and significant linkage disequilibrium occurred between HeSNP24 and HeSNP38 (P < 0.01). The present study can provide an effective tool for population study and genetic breeding.
部门归属[Qu, Hongyue; Luo, Wei; Lin, Qiang] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Qu, Hongyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; LMB
关键词Hippocampus Erectus Snp Transcriptome Genetic Diversity
学科领域Biodiversity & Conservation ; Genetics & Heredity
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15482
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Lin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
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GB/T 7714
Qu, Hongyue,Luo, Wei,Lin, Qiang,et al. Development of SNP markers in lined seahorse (Hippocampus erectus) based on transcriptome sequencing[J]. CONSERVATION GENETICS RESOURCES,2016,8(1):2016-01-04.
APA Qu, Hongyue,Luo, Wei,Lin, Qiang,&Lin, Q .(2016).Development of SNP markers in lined seahorse (Hippocampus erectus) based on transcriptome sequencing.CONSERVATION GENETICS RESOURCES,8(1),2016-01-04.
MLA Qu, Hongyue,et al."Development of SNP markers in lined seahorse (Hippocampus erectus) based on transcriptome sequencing".CONSERVATION GENETICS RESOURCES 8.1(2016):2016-01-04.
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