Characterization of a Metagenome-Derived beta-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol
Mai, Zhimao; Su, Hongfei; Zhang, Si; Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊CATALYSTS
卷号6期号:3页码:35
摘要For the beneficial pharmacological properties of resveratrol, there is increasingly interest in enzymatic conversion of polydatin to resveratrol. The metagenomic technique provides an effective strategy for mining novel polydatin-hydrolysis enzymes from uncultured microorganisms. In this study, a metagenomic library of mangrove soil was constructed and a novel -glucosidase gene MlBgl was isolated. The deduced amino acid sequences of MlBgl showed the highest identity of 64% with predicted -glucosidase in the GenBank database. The gene was cloned and overexpressed in Escherichia coli BL21(DE3). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay demonstrated the purified recombinant -glucosidase r-MlBgl with a molecular weight approximately of 71 kDa. The optimal pH and temperature of purified recombinant r-MlBgl were 7.0 and 40 degrees C, respectively. r-MlBgl could hydrolyze polydatin effectively. The k(cat) and k(cat)/K-m values for polydatin were 989 s(-1) and 1476 mM(-1)s(-1), respectively. These properties suggest that -r-MlBgl has potential application in the enzymatic conversion of polydatin to resveratrol for further study.
部门归属[Mai, Zhimao; Su, Hongfei; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; LMB
关键词Beta-glucosidase Mangrove Soil Metagenomic Library Polydatin Resveratrol
学科领域Chemistry
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15483
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
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Mai, Zhimao,Su, Hongfei,Zhang, Si,et al. Characterization of a Metagenome-Derived beta-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol[J]. CATALYSTS,2016,6(3):35.
APA Mai, Zhimao,Su, Hongfei,Zhang, Si,&Zhang, S .(2016).Characterization of a Metagenome-Derived beta-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol.CATALYSTS,6(3),35.
MLA Mai, Zhimao,et al."Characterization of a Metagenome-Derived beta-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol".CATALYSTS 6.3(2016):35.
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