Fish TRIM39 regulates cell cycle progression and exerts its antiviral function against iridovirus and nodavirus
Wang, Wei; Huang, Youhua; Yu, Yepin; Yang, Ying; Xu, Meng; Chen, Xiuli; Ni, Songwei; Qin, Qiwei; Huang, Xiaohong; Qin, QW; Huang, XH (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号50页码:2016-01-10
摘要The tripartite motif (TRIM)-containing proteins exert important immune regulatory roles through regulating different signaling pathways in response to different stimuli. TRIM39, a member of the TRIM family, is a RING domain-containing E3 ubiquitin ligase which could regulate cell cycle progression and apoptosis. However, the antiviral activity of TRIM39 is not explored. Here, a TRIM39 homolog from grouper, Epinephelus coioides (EcTRIM39) was cloned, and its effects on cell cycle progression and fish virus replication were investigated. The full-length EcTRIM39 cDNA was composed of 2535 bp and encoded a polypeptide of 543 amino acids with 70% identity with TRIM39 homologs from bicolor damselfish. Amino acid alignment analysis indicated that EcTRIM39 contained a RING finger, B-box and SPRY domain. Expression profile analysis revealed that EcTRIM39 was abundant in intestine, spleen and skin. Upon different stimuli in vivo, the EcTRIM39 transcript was obviously up-regulated after challenging with Singapore grouper iridovirus (SGIV), and polyinosinic-polycytidylic acid (poly I:C). Using fluorescence microscopy, we found that EcTRIM39 localized in the cytoplasm and formed aggregates in grouper spleen (GS) cells. The ectopic expression of EcTRIM39 in vitro affected the cell cycle progression via mediating G1/S transition. Moreover, the RING domain was essential for its accurate localization and effect on cell cycle. In addition, overexpression of EcTRIM39 significantly inhibited viral gene transcription of SGIV and red-spotted grouper nervous necrosis virus (RGNNV) in vitro, and the mutant of RING exerted the opposite effect. Together, our results demonstrated that fish TRIM39 not only regulated the cell cycle progression, but also acted as an important regulator of fish innate immune response against viruses. (C) 2016 Elsevier Ltd. All rights reserved.
部门归属[Wang, Wei; Huang, Youhua; Yu, Yepin; Yang, Ying; Chen, Xiuli; Ni, Songwei; Qin, Qiwei; Huang, Xiaohong] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Wei; Yu, Yepin; Yang, Ying; Chen, Xiuli; Ni, Songwei] Univ Chinese Acad Sci, Beijing, Peoples R China; [Xu, Meng] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China ; LMB
关键词Trim39 Grouper Cell Cycle Antiviral Viral Replication
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15485
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Qin, QW; Huang, XH (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
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Wang, Wei,Huang, Youhua,Yu, Yepin,et al. Fish TRIM39 regulates cell cycle progression and exerts its antiviral function against iridovirus and nodavirus[J]. FISH & SHELLFISH IMMUNOLOGY,2016,50:2016-01-10.
APA Wang, Wei.,Huang, Youhua.,Yu, Yepin.,Yang, Ying.,Xu, Meng.,...&Huang, XH .(2016).Fish TRIM39 regulates cell cycle progression and exerts its antiviral function against iridovirus and nodavirus.FISH & SHELLFISH IMMUNOLOGY,50,2016-01-10.
MLA Wang, Wei,et al."Fish TRIM39 regulates cell cycle progression and exerts its antiviral function against iridovirus and nodavirus".FISH & SHELLFISH IMMUNOLOGY 50(2016):2016-01-10.
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