Molecular clone and characterization of c-Jun N-terminal kinases 2 from orange-spotted grouper, Epinephelus coioides
Guo, Minglan; Wei, Jingguang; Zhou, Yongcan; Qin, Qiwei; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号49页码:355-363
摘要c-Jun N-terminal kinase 2 (JNK2) is a multifunctional mitogen-activated protein kinases involving in cell differentiation and proliferation, apoptosis, immune response and inflammatory conditions. In this study, we reported a new JNK2 (Ec-JNK2) derived from orange-spotted grouper, Epinephelus coioides. The full-length cDNA of Ec-INK2 was 1920 bp in size, containing a 174 bp 5'-untranslated region (IJTR), 483 bp 3'-UTR, and a 1263 bp open reading frame (ORF), which encoded a putative protein of 420 amino acids. The deduced protein sequence of Ec-JNK2 contained a conserved Thr-Pro-Tyr (WY) motif in the domain of serine/threonine protein kinase (S-TKc). Ec-JNK2 has been found to involve in the immune response to pathogen challenges in vivo, and the infection of Singapore grouper iridovirus (SGIV) in vitro. Immunofluorescence staining showed that Ec-JNK2 was localized in the cytoplasm of grouper spleen (GS) cells, and moved to the nucleus after infecting with SGIV. Ec-JNK2 distributed in all immune-related tissues examined. After challenging with lipopolysaccharide (LPS), SGIV and polyriboinosinic polyribocytidylic acid (poly I:C), the mRNA expression of Ec-JNK2 was significantly (P < 0.01) up-regulated in juvenile orange-spotted grouper. Over-expressing Ec-JNK2 in fathead minnow (FHM) cells increased the SGIV infection and replication, while over-expressing the dominant-negative Ec-JNK2 Delta 181-183 mutant decreased it. These results indicated that Ec-JNK2 could be an important molecule in the successful infection and evasion of SGIV. (C) 2015 Elsevier Ltd. All rights reserved.
部门归属[Guo, Minglan; Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Guo, Minglan; Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Zhou, Yongcan] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China ; LMB
关键词C-jun N-terminal Kinases 2 Epinephelus Coioides Molecular Clone Singapore Grouper Iridovirus (Sgiv)
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15500
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
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GB/T 7714
Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,et al. Molecular clone and characterization of c-Jun N-terminal kinases 2 from orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2016,49:355-363.
APA Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,Qin, Qiwei,&Qin, QW .(2016).Molecular clone and characterization of c-Jun N-terminal kinases 2 from orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,49,355-363.
MLA Guo, Minglan,et al."Molecular clone and characterization of c-Jun N-terminal kinases 2 from orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 49(2016):355-363.
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