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Crustacean cardioactive peptide (CCAP) of the Pacific white shrimp (Litopenaeus vannamei): Molecular characterization and its potential roles in osmoregulation and freshwater tolerance
Chen, Ting; Ren, Chunhua; Wang, Yanhong; Gao, Yan; Wong, Nai-Kei; Zhang, Lvping; Hu, Chaoqun; Ren, CH (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationAQUACULTURE
Volume451Pages:405-412
AbstractOsmoregulation is one of the most fundamental and delicately controlled processes in euryhaline marine species like penaeid shrimp. Here we first report a cDNA encoding for the crustacean cardioactive peptide (CCAP) precursor cloned from the intestine of Pacific white shrimp, Litopenaeus vannamei, a prominent commercial culture species. The CCAP mature peptide (PFCNAFTGC-NH2) is generated by post-translational processing and found to be highly comparable with its counterparts in insects and other crustaceans. Semi-quantitative RT-PCR reveals that L. vannamei CCAP (Lv-CCAP) mRNA is predominantly expressed in the brain and intestine. However, only the expression level of intestine-derived Lv-CCAP mRNA showed responsiveness to salinity changes in culture. The inducible expressed Lv-CCAP mRNA by transfer to high-salinity condition was continuous, while mRNA expression stimulated by transfer to low-salinity condition was transient. Functionally, the stimulatory effects of CCAP on heart rates in the shrimp cultured in low-and normal-salinity conditions were confirmed. Furthermore, injection of CCAP could up-regulate the freshwater tolerance of shrimp by increasing their survival rates. Our study, as a whole, provides new insights into hormone-regulated crustacean osmostasis and may prove instructive to the inland freshwater penaeid shrimp aquaculture. State of relevance It proves instructive to freshwater shrimp aquaculture. (C) 2015 Elsevier B.V. All rights reserved.
Department[Chen, Ting; Ren, Chunhua; Wang, Yanhong; Gao, Yan; Zhang, Lvping; Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Ting; Ren, Chunhua; Hu, Chaoqun] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China; [Wong, Nai-Kei] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China ; LMB
KeywordCrustacean Cardioactive Peptide Molecular Cloning Heart Rate Osmoregulation Salinity Stress Freshwater Tolerance
Subject AreaFisheries ; Marine & Freshwater Biology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15506
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorRen, CH (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Chen, Ting,Ren, Chunhua,Wang, Yanhong,et al. Crustacean cardioactive peptide (CCAP) of the Pacific white shrimp (Litopenaeus vannamei): Molecular characterization and its potential roles in osmoregulation and freshwater tolerance[J]. AQUACULTURE,2016,451:405-412.
APA Chen, Ting.,Ren, Chunhua.,Wang, Yanhong.,Gao, Yan.,Wong, Nai-Kei.,...&Ren, CH .(2016).Crustacean cardioactive peptide (CCAP) of the Pacific white shrimp (Litopenaeus vannamei): Molecular characterization and its potential roles in osmoregulation and freshwater tolerance.AQUACULTURE,451,405-412.
MLA Chen, Ting,et al."Crustacean cardioactive peptide (CCAP) of the Pacific white shrimp (Litopenaeus vannamei): Molecular characterization and its potential roles in osmoregulation and freshwater tolerance".AQUACULTURE 451(2016):405-412.
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