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Characterization of a Main Extracellular Matrix Autoenzyme from the Dermis of Sea Cucumber Stichopus monotuberculatus: Collagenase
Zhong, Ming; Hu, Chaoqun; Ren, Chunhua; Luo, Xing; Cai, Yiming; Hu, CQ (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationINTERNATIONAL JOURNAL OF FOOD PROPERTIES
Volume19Issue:11Pages:2495-2509
AbstractSea cucumber is easy to undergo autolysis, causing serious economic loss in the aquaculture, fishery, and food processing. However, research on the autolysis of sea cucumber is limited. In the present study, the autoenzyme that could hydrolyze the structural protein of body wall was identified and characterized in sea cucumber Stichopus monotuberculatus. Although the main edible part of sea cucumber was the body wall, its microstructure was shown to be almost composed of collagen fibers belonging to extracellular matrix, indicating that the body wall of S. monotuberculatus belonged to dermis tissue. Starting from this perspective, crude collagen fibrils from dermis of S. monotuberculatus was isolated, and pepsin solubilized collagen was also obtained by hydrolyzing collagen telopeptides from crude collagen fibrils. Crude collagen fibrils and pepsin solubilized collagen were then acting as substrates, respectively, to identify dermis autoenzyme which had hydrolytic activity on dermis collagen. After that, a collagenase of 45 kDa was identified with the method of collagen zymograph, and the content of soluble protein was suppressed by collagenase inhibitor significantly in the autolysis of dermis. Finally, the effect of environmental conditions on collagenase activity was studied, results showed its best activity was at 40 degrees C and pH 8, and divalent metal ion Mn2+ was essential for its activity. As a whole, our results showed that the dermis of sea cucumber was composed of collagen fibers, and collagenase was the main enzyme resulting in autolysis of sea cucumber.
Department[Zhong, Ming; Hu, Chaoqun; Ren, Chunhua; Luo, Xing; Cai, Yiming] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol Chinese A, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou, Guangdong, Peoples R China; [Zhong, Ming; Luo, Xing; Cai, Yiming] Univ Chinese Acad Sci, Beijing, Peoples R China; [Zhong, Ming] Jiaying Univ, Meizhou, Peoples R China ; LMB
KeywordAutolysis Dermis Collagen Collagenase Sea Cucumber Stichopus Monotuberculatus
Subject AreaFood Science & Technology
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15517
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorHu, CQ (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Zhong, Ming,Hu, Chaoqun,Ren, Chunhua,et al. Characterization of a Main Extracellular Matrix Autoenzyme from the Dermis of Sea Cucumber Stichopus monotuberculatus: Collagenase[J]. INTERNATIONAL JOURNAL OF FOOD PROPERTIES,2016,19(11):2495-2509.
APA Zhong, Ming,Hu, Chaoqun,Ren, Chunhua,Luo, Xing,Cai, Yiming,&Hu, CQ .(2016).Characterization of a Main Extracellular Matrix Autoenzyme from the Dermis of Sea Cucumber Stichopus monotuberculatus: Collagenase.INTERNATIONAL JOURNAL OF FOOD PROPERTIES,19(11),2495-2509.
MLA Zhong, Ming,et al."Characterization of a Main Extracellular Matrix Autoenzyme from the Dermis of Sea Cucumber Stichopus monotuberculatus: Collagenase".INTERNATIONAL JOURNAL OF FOOD PROPERTIES 19.11(2016):2495-2509.
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